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Methods in Molecular Biology

Atomic Force Microscopy

Methods and Protocols

Editors: Santos, Nuno, Carvalho, Filomena (Eds.)

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  • Includes cutting-edge lab techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8894-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8893-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Artifacts and Practical Issues in Atomic Force Microscopy

    Pages 3-28

    Eaton, Peter (et al.)

  • Quantitative Analysis of Structure and Dynamics in AFM Images of Lipid Membranes

    Pages 29-44

    Connell, Simon D. (et al.)

  • Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy

    Pages 45-59

    Nasrallah, Hussein (et al.)

  • Investigating the Nanodomain Organization of Rhodopsin in Native Membranes by Atomic Force Microscopy

    Pages 61-74

    Senapati, Subhadip (et al.)

  • Analysis of DNA–Protein Complexes by Atomic Force Microscopy Imaging: The Case of TRF2–Telomeric DNA Wrapping

    Pages 75-97

    Pisano, Sabrina (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8894-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8893-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Atomic Force Microscopy
Book Subtitle
Methods and Protocols
Editors
  • Nuno Santos
  • Filomena Carvalho
Series Title
Methods in Molecular Biology
Series Volume
1886
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8894-5
DOI
10.1007/978-1-4939-8894-5
Hardcover ISBN
978-1-4939-8893-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 372
Number of Illustrations
16 b/w illustrations, 87 illustrations in colour
Topics