Methods in Molecular Biology

Systems Chemical Biology

Methods and Protocols

Editors: Ziegler, Slava, Waldmann, Herbert (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and advice from the experts
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  • ISBN 978-1-4939-8891-4
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Hardcover $179.99
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About this book

This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets. The chapters in this book discuss topics such as disease-relevant models for chemical biology studies, target engagement using cellular thermal shift assay or bioluminescence resonance energy transfer; visualization of bio-active small molecules Raman microscopy; (phospho-)proteomics and transcriptomics for mode-of-action studies, CRISPR/Cas9-based chemogenomic profiling in mammalian cells; predicting drug interactions using computational approaches; comparison of compound-induced profiles using high-content imaging or cancer cell line panels and web-based tools for polypharmacology prediction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Systems Chemical Biology: Methods and Protocols is a valuable resource for novice or expert scientists and researchers trying to initiate or continue their chemical biology studies at a systems level.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • PREDECT Protocols for Complex 2D/3D Cultures

    Vidic, Suzana (et al.)

    Pages 1-20

  • Phenotypic Screening Using Mouse and Human Stem Cell-Based Models of Neuroinflammation and Gene Expression Analysis to Study Drug Responses

    Abo-Rady, Masin (et al.)

    Pages 21-43

  • Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer

    Robers, Matthew B. (et al.)

    Pages 45-71

  • Target Engagement of Small Molecules: Thermal Profiling Approaches on Different Levels

    Reckzeh, Elena S. (et al.)

    Pages 73-98

  • Visualizing Bioactive Small Molecules by Alkyne Tagging and Slit-Scanning Raman Microscopy

    Ando, Jun (et al.)

    Pages 99-114

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8891-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8890-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Systems Chemical Biology
Book Subtitle
Methods and Protocols
Editors
  • Slava Ziegler
  • Herbert Waldmann
Series Title
Methods in Molecular Biology
Series Volume
1888
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8891-4
DOI
10.1007/978-1-4939-8891-4
Hardcover ISBN
978-1-4939-8890-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 315
Number of Illustrations
22 b/w illustrations, 43 illustrations in colour
Topics