Systems Chemical Biology
Methods and Protocols
Editors: Ziegler, Slava, Waldmann, Herbert (Eds.)
Free Preview- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key notes and advice from the experts
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- About this book
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This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets. The chapters in this book discuss topics such as disease-relevant models for chemical biology studies, target engagement using cellular thermal shift assay or bioluminescence resonance energy transfer; visualization of bio-active small molecules Raman microscopy; (phospho-)proteomics and transcriptomics for mode-of-action studies, CRISPR/Cas9-based chemogenomic profiling in mammalian cells; predicting drug interactions using computational approaches; comparison of compound-induced profiles using high-content imaging or cancer cell line panels and web-based tools for polypharmacology prediction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Systems Chemical Biology: Methods and Protocols is a valuable resource for novice or expert scientists and researchers trying to initiate or continue their chemical biology studies at a systems level.
- Table of contents (16 chapters)
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PREDECT Protocols for Complex 2D/3D Cultures
Pages 1-20
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Phenotypic Screening Using Mouse and Human Stem Cell-Based Models of Neuroinflammation and Gene Expression Analysis to Study Drug Responses
Pages 21-43
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Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer
Pages 45-71
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Target Engagement of Small Molecules: Thermal Profiling Approaches on Different Levels
Pages 73-98
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Visualizing Bioactive Small Molecules by Alkyne Tagging and Slit-Scanning Raman Microscopy
Pages 99-114
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Systems Chemical Biology
- Book Subtitle
- Methods and Protocols
- Editors
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- Slava Ziegler
- Herbert Waldmann
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1888
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8891-4
- DOI
- 10.1007/978-1-4939-8891-4
- Hardcover ISBN
- 978-1-4939-8890-7
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 315
- Number of Illustrations
- 22 b/w illustrations, 43 illustrations in colour
- Topics
