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Cancer Immunosurveillance

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Alejandro López-Soto

    1. Departamento de Biología Funcional, Inmunología, Universidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Oviedo, Spain

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  2. Alicia R. Folgueras

    1. Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Oviedo, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1884)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer

    • Willem H. Zoutman, Rogier J. Nell, Pieter A. van der Velden
    Pages 1-14

  3. An Economical, Quantitative, and Robust Protocol for High-Throughput T Cell Receptor Sequencing from Tumor or Blood

    • Imran Uddin, Kroopa Joshi, Theres Oakes, James M. Heather, Charles Swanton, on behalf of the TRACERx consortium et al.
    Pages 15-42

  4. Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells

    • Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, Jianxun Song
    Pages 43-55

  5. Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Antitumor Effects In Vivo

    • Akshat Sharma, Nicholas A. Zumwalde, Jenny E. Gumperz
    Pages 57-72

  6. Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors

    • Yong Yu, Pengtao Liu
    Pages 73-86

  7. Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance

    • Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, Massimo Vitale
    Pages 87-117

  8. CD107a Degranulation Assay to Evaluate Immune Cell Antitumor Activity

    • Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, Alejandro López-Soto
    Pages 119-130

  9. A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anticancer Immune Responses In Vitro

    • Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González, Alejandro López-Soto
    Pages 131-139

  10. Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency

    • Vidhya R. Nair, Srinivas Malladi
    Pages 141-150

  11. Tumor-Associated Macrophage Isolation and In Vivo Analysis of Their Tumor-Promoting Activity

    • Nicholas M. Clark, Paula D. Bos
    Pages 151-160

  12. Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo

    • Magali Dupont, Christian A. J. Vosshenrich
    Pages 161-176

  13. Determination and Isolation of Immune Populations from Brain Tumor Microenvironments

    • Javier Godino, Alberto J. Schuhmacher
    Pages 177-188

  14. Mouse Model of Colitis-Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells

    • Llipsy Santiago, Marta Castro, Julián Pardo, Maykel Arias
    Pages 189-202

  15. Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry

    • Shelly Kalaora, Yardena Samuels
    Pages 203-214

  16. Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways

    • Cherie’ R. Scurrah, Alan J. Simmons, Ken S. Lau
    Pages 215-229

  17. Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation

    • Marina Wierz, Sandrine Pierson, Ernesto Gargiulo, Coralie Guerin, Etienne Moussay, Jerome Paggetti
    Pages 231-245

  18. Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion

    • Chiara Nicolazzo, Angela Gradilone, Guido Carpino, Paola Gazzaniga, Cristina Raimondi
    Pages 247-258

  19. Quantitative Identification of Senescent Cells in Cancer

    • Anat Biran, Ziv Porat, Valery Krizhanovsky
    Pages 259-267

  20. Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance

    • Dirk Zboralski, Anna Frömming
    Pages 269-281
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Keywords

About this book

This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field.

Editors and Affiliations

  • Departamento de Biología Funcional, Inmunología, Universidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Oviedo, Spain

    Alejandro López-Soto

  • Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Oviedo, Spain

    Alicia R. Folgueras

Bibliographic Information

  • Book Title: Cancer Immunosurveillance

  • Book Subtitle: Methods and Protocols

  • Editors: Alejandro López-Soto, Alicia R. Folgueras

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8885-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8884-6Published: 22 November 2018

  • eBook ISBN: 978-1-4939-8885-3Published: 21 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 363

  • Number of Illustrations: 13 b/w illustrations, 63 illustrations in colour

  • Topics: Cancer Research

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