Methods in Molecular Biology

Cancer Immunosurveillance

Methods and Protocols

Editors: Lopez-Soto, Alejandro, Folgueras, Alicia R. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-8885-3
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Hardcover $219.99
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About this book

This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer

    Zoutman, Willem H. (et al.)

    Pages 1-14

  • An Economical, Quantitative, and Robust Protocol for High-Throughput T Cell Receptor Sequencing from Tumor or Blood

    Uddin, Imran (et al.)

    Pages 15-42

  • Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells

    Chen, Xiaoniao (et al.)

    Pages 43-55

  • Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Antitumor Effects In Vivo

    Sharma, Akshat (et al.)

    Pages 57-72

  • Usage of Single-Cell RNA Sequencing to Unveil Immune Lymphoid Cell Precursors

    Yu, Yong (et al.)

    Pages 73-86

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8885-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8884-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cancer Immunosurveillance
Book Subtitle
Methods and Protocols
Editors
  • Alejandro Lopez-Soto
  • Alicia R. Folgueras
Series Title
Methods in Molecular Biology
Series Volume
1884
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8885-3
DOI
10.1007/978-1-4939-8885-3
Hardcover ISBN
978-1-4939-8884-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 363
Number of Illustrations
13 b/w illustrations, 63 illustrations in colour
Topics