Skip to main content
SpringerLink
Log in

Skin Stem Cells

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes fully revised and all new cutting-edge methods and protocols for the study of skin stem cell research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1879)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging

    • Olivia Farrelly, Paola Kuri, Panteleimon Rompolas
    Pages 1-14

  3. Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells

    • Daniel Haensel, Melissa A. McNeil, Xing Dai
    Pages 15-29

  4. Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction

    • Frédéric Auvré, Julien Coutier, Michèle T. Martin, Nicolas O. Fortunel
    Pages 31-41

  5. Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction

    • Gaëtan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, Sylvain L. Guérin
    Pages 43-73

  6. Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells

    • Wenbo Wu, Ting Chen
    Pages 75-86

  7. Isolation and Characterization of Cutaneous Epithelial Stem Cells

    • Stephanie R. Gillespie, David M. Owens
    Pages 87-99

  8. Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

    • Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, Alexandra P. Marques
    Pages 101-110

  9. Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells

    • Edwige Roy, Kiarash Khosrotehrani
    Pages 111-118

  10. Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis

    • Stefano Sol, Dario Antonini, Caterina Missero
    Pages 119-132

  11. Isolation and Cultivation of Epidermal (Stem) Cells

    • Xusheng Wang, Shiyang Dong, Yaojiong Wu
    Pages 133-138

  12. One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen

    • Hua Qian, Xue Leng, Jie Wen, Qian Zhou, Xin Xu, Xunwei Wu
    Pages 139-148

  13. Isolation and Cultivation of Skin-Derived Precursors

    • Xiaoxiao Wang, Shiyang Dong, Yaojiong Wu
    Pages 149-152

  14. Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

    • Petek Korkusuz, Sevil Köse, Nilgün Yersal, Selin Önen
    Pages 153-163

  15. Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor

    • Meilang Xue, Suat Dervish, Christopher J. Jackson
    Pages 165-174

  16. Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair

    • Mahmut Parmaksiz, AyÅŸe Eser Elçin, YaÅŸar Murat Elçin
    Pages 175-185

  17. Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet

    • Lei Chen, Daniel Radke, Shaohai Qi, Feng Zhao
    Pages 187-200

  18. Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

    • Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, Amir Bajouri
    Pages 201-210

  19. Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

    • Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, Amir Bajouri
    Pages 211-219

  20. Skin Wound Healing: Refractory Wounds and Novel Solutions

    • Gabriel M. Virador, Lola de Marcos, Victoria M. Virador
    Pages 221-241
Back to top

Keywords

About this book

This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells.

Editors and Affiliations

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Skin Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8870-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8869-3Published: 30 January 2019

  • eBook ISBN: 978-1-4939-8870-9Published: 29 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 463

  • Number of Illustrations: 29 b/w illustrations, 76 illustrations in colour

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation