Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1877)
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Table of contents (21 protocols)
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Front Matter
Keywords
About this book
This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health and disease; evaluating cellular dependencies to specific BCL-2 family proteins; flow-cytometry-based methods for measuring BCL-2 proteins and mitochondrial-based cell death; measuring activity and interactions of BCL-2 family proteins in the presence of mitochondria, artificial membranes or yeast; conformational activation and oligomerization of pro-apoptotic proteins BAX and BAK leading to cytochrome c release and apoptosis; structural and biophysical studies in solution and lipid vesicles using nuclear magnetic resonance, cryo-electron microscopy, fluorescence microscopy and electron paramagnetic resonance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, BCL-2 Family Proteins: Methods and Protocols is a valuable resource to inspire and encourage novice and established scientists to further their research and make new discoveries in this exciting field.
Editors and Affiliations
Bibliographic Information
Book Title: BCL-2 Family Proteins
Book Subtitle: Methods and Protocols
Editors: Evripidis Gavathiotis
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8861-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8860-0Published: 07 December 2018
eBook ISBN: 978-1-4939-8861-7Published: 07 December 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 360
Number of Illustrations: 30 b/w illustrations, 66 illustrations in colour
Topics: Protein Science