Methods in Molecular Biology

BCL-2 Family Proteins

Methods and Protocols

Editors: Gavathiotis, Evripidis (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-8861-7
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Hardcover $279.99
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About this book

This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health and disease; evaluating cellular dependencies to specific BCL-2 family proteins; flow-cytometry-based methods for measuring BCL-2 proteins and mitochondrial-based cell death; measuring activity and  interactions of BCL-2 family proteins in the presence of mitochondria, artificial membranes or yeast; conformational activation and oligomerization of pro-apoptotic proteins BAX and BAK leading to cytochrome c release and apoptosis; structural and biophysical studies in solution and lipid vesicles using nuclear magnetic resonance, cryo-electron microscopy, fluorescence microscopy and electron paramagnetic resonance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, BCL-2 Family Proteins: Methods and Protocols is a valuable resource to inspire and encourage novice and established scientists to further their research and make new discoveries in this exciting field.


Table of contents (21 chapters)

  • Overview of BCL-2 Family Proteins and Therapeutic Potentials

    Huska, Jason D. (et al.)

    Pages 1-21

  • Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins

    Aouacheria, Abdel (et al.)

    Pages 23-43

  • Application of Mito-Priming to Generate BCL-2 Addicted Cells

    Lopez, Jonathan (et al.)

    Pages 45-60

  • BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies

    Fraser, Cameron (et al.)

    Pages 61-76

  • Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP)

    Ludwig, Lindsey M. (et al.)

    Pages 77-91

Buy this book

eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8861-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-1-4939-8860-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
BCL-2 Family Proteins
Book Subtitle
Methods and Protocols
Editors
  • Evripidis Gavathiotis
Series Title
Methods in Molecular Biology
Series Volume
1877
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8861-7
DOI
10.1007/978-1-4939-8861-7
Hardcover ISBN
978-1-4939-8860-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 360
Number of Illustrations
30 b/w illustrations, 66 illustrations in colour
Topics