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Methods in Molecular Biology

Microinjection

Methods and Protocols

Editors: Liu, Chengyu, Du, Yubin (Eds.)

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  • Includes cutting-edge techniques for the study of microinjection and its purposes
  • Focuses on mouse models for half of the book, with the other half centered on a wide variety of other models
  • Contains key implementation advice from the experts
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eBook $119.00
price for USA in USD
  • ISBN 978-1-4939-8831-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8830-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $159.99
price for USA in USD
  • ISBN 978-1-4939-9398-7
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
About this book

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Microinjection and Micromanipulation: A Historical Perspective

    Pages 1-16

    Xu, Wenhao

  • Production of Transgenic Mice by Pronuclear Microinjection

    Pages 17-41

    Pu, Xin-an (et al.)

  • Transgene Recombineering in Bacterial Artificial Chromosomes

    Pages 43-69

    Zeidler, Michael G. (et al.)

  • Using TARGATT™ Technology to Generate Site-Specific Transgenic Mice

    Pages 71-86

    Chen-Tsai, Ruby Yanru

  • Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method

    Pages 87-98

    Sato, Takuya (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-4939-8831-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8830-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $159.99
price for USA in USD
  • ISBN 978-1-4939-9398-7
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Microinjection
Book Subtitle
Methods and Protocols
Editors
  • Chengyu Liu
  • Yubin Du
Series Title
Methods in Molecular Biology
Series Volume
1874
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8831-0
DOI
10.1007/978-1-4939-8831-0
Hardcover ISBN
978-1-4939-8830-3
Softcover ISBN
978-1-4939-9398-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 540
Number of Illustrations
38 b/w illustrations, 96 illustrations in colour
Topics