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Methods in Molecular Biology

Epitranscriptomics

Methods and Protocols

Editors: Wajapeyee, Narendra, Gupta, Romi (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume provides readers with the latest technologies to study changes in the epitranscriptome. The protocols described in this book explore both targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and detection and quantification of pseudouridine in RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Epitranscriptomics: Methods and Protocols is an important resource for both expert and novice scientists who are interested in learning more about this field.


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine

    Pages 1-21

    Trixl, Lukas (et al.)

  • Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular Beacons

    Pages 23-39

    Chen, Mingming (et al.)

  • Visualization of Xist Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System

    Pages 41-50

    Waśko, Urszula (et al.)

  • HAMR: High-Throughput Annotation of Modified Ribonucleotides

    Pages 51-67

    Vandivier, Lee E. (et al.)

  • High-Resolution Mapping of N 6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq)

    Pages 69-79

    Hsu, Phillip J. (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-4939-8808-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8807-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Epitranscriptomics
Book Subtitle
Methods and Protocols
Editors
  • Narendra Wajapeyee
  • Romi Gupta
Series Title
Methods in Molecular Biology
Series Volume
1870
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8808-2
DOI
10.1007/978-1-4939-8808-2
Hardcover ISBN
978-1-4939-8807-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 298
Number of Illustrations
16 b/w illustrations, 41 illustrations in colour
Topics

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