Brain Tumor Stem Cells
Methods and Protocols
Editors: Singh, Sheila K., Venugopal, Chitra (Eds.)
Free Preview- Includes cutting-edge techniques for the study of brain tumor stem cell research
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocols serves as an ideal guide for researchers seeking to better understand the complexities of brain cancer. - Reviews
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“Sheila K. Singh and Chitra Venugopal have developed a concise, systematic, and practical book; an ideal read for brain cancer researchers. … this book is recommended for anyone who strives for proficiency in biophysiology of cancer stem cells and hopes to discover novel therapeutic and treatment strategies for patients affected by devastating CNS disease.” (Angela M. Bohnen, Neurosurgery, Vol. 85 (4), October, 2019)
- Table of contents (20 chapters)
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Introduction to Brain Tumor Stem Cells
Pages 1-9
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Isolation and Culture of Glioblastoma Brain Tumor Stem Cells
Pages 11-21
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Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines
Pages 23-36
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Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors
Pages 37-56
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Detecting Stem Cell Marker Expression Using the NanoString nCounter System
Pages 57-67
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Brain Tumor Stem Cells
- Book Subtitle
- Methods and Protocols
- Editors
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- Sheila K. Singh
- Chitra Venugopal
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1869
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8805-1
- DOI
- 10.1007/978-1-4939-8805-1
- Hardcover ISBN
- 978-1-4939-8804-4
- Softcover ISBN
- 978-1-4939-9394-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 254
- Number of Illustrations
- 6 b/w illustrations, 34 illustrations in colour
- Topics
