Brain Tumor Stem Cells

1. Front Matter

   Pages i-xii

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2. Introduction to Brain Tumor Stem Cells

   • Nicolas Yelle, David Bakhshinyan, Chitra Venugopal, Sheila K. Singh

   Pages 1-9

3. Isolation and Culture of Glioblastoma Brain Tumor Stem Cells

   • Charles Chesnelong, Ian Restall, Samuel Weiss

   Pages 11-21

4. Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines

   • Sara Badodi, Silvia Marino, Loredana Guglielmi

   Pages 23-36

5. Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors

   • Vijay Ramaswamy, Michael D. Taylor

   Pages 37-56

6. Detecting Stem Cell Marker Expression Using the NanoString nCounter System

   • Scott Ryall, Anthony Arnoldo, Javal Sheth, Sheila K. Singh, Cynthia Hawkins

   Pages 57-67

7. Flow Cytometric Analysis of Brain Tumor Stem Cells

   • Minomi K. Subapanditha, Ashley A. Adile, Chitra Venugopal, Sheila K. Singh

   Pages 69-77

8. In Vitro Self-Renewal Assays for Brain Tumor Stem Cells

   • Mathieu Seyfrid, David Bobrowski, David Bakhshinyan, Nazanin Tatari, Chitra Venugopal, Sheila K. Singh

   Pages 79-84

9. Differentiation of Brain Tumor Initiating Cells

   • Michelle M. Kameda-Smith, Minomi K. Subapanditha, Sabra K. Salim, Chitra Venugopal, Sheila K. Singh

   Pages 85-91

10. The Study of Brain Tumor Stem Cell Migration

    • Montserrat Lara-Velazquez, Rawan Al-kharboosh, Luis Prieto, Paula Schiapparelli, Alfredo Quiñones-Hinojosa

    Pages 93-104

11. The Study of Brain Tumor Stem Cell Invasion

    • Rawan Al-kharboosh, Montserrat Lara-Velazquez, Luis Prieto, Rachel Sarabia-Estrada, Alfredo Quiñones-Hinojosa

    Pages 105-116

12. Cell Cycle Dynamics in Glioma Cancer Stem Cells

    • Ingrid Qemo, Lisa A. Porter

    Pages 117-126

13. Embryonic Stem Cell Models of Human Brain Tumors

    • Ludivine Coudière Morrison, Nazanin Tatari, Tamra E. Werbowetski-Ogilvie

    Pages 127-142

14. Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory

    • Hunter McColl, Jamie L. Zagozewski, David D. Eisenstat

    Pages 143-153

15. EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF

    • Amy X. Hu, Jarrett J. Adams, Parvez Vora, Maleeha Qazi, Sheila K. Singh, Jason Moffat et al.

    Pages 155-168

16. Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells

    • Michael Aregger, Megha Chandrashekhar, Amy Hin Yan Tong, Katherine Chan, Jason Moffat

    Pages 169-188

17. In Vitro Assays for Screening Small Molecules

    • Ashley A. Adile, David Bakhshinyan, Chitra Venugopal, Sheila K. Singh

    Pages 189-196

18. Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma

    • Elena Binda, Alberto Visioli, Nadia Trivieri, Angelo Luigi Vescovi

    Pages 197-205

19. Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci

    • Jon D. Larson, Suzanne J. Baker

    Pages 207-230

20. In Vivo Murine Models of Brain Metastasis

    • Mohini Singh, Neil Savage, Sheila K. Singh

    Pages 231-238

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocolsserves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.

• Brain cancer
• BTSC research
• Human cancer stem cell (CSC) populations
• Therapeutic options
• Glioblastoma

“Sheila K. Singh and Chitra Venugopal have developed a concise, systematic, and practical book; an ideal read for brain cancer researchers. … this book is recommended for anyone who strives for proficiency in biophysiology of cancer stem cells and hopes to discover novel therapeutic and treatment strategies for patients affected by devastating CNS disease.” (Angela M. Bohnen, Neurosurgery, Vol. 85 (4), October, 2019)

• Stem Cell and Cancer Research Institute, McMaster University, Hamilton, Canada

  Sheila K. Singh, Chitra Venugopal

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