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Zinc Finger Proteins

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Jia Liu

    1. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China

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  • Includes cutting-edge methods and protocols for the study of zinc finger proteins
  • Provides step-by-step detail essential for reproducible results
  • Contains notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1867)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
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  2. Basic Biology and Engineering of ZFPs

    1. Front Matter

      Pages 1-1
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    2. PRDM14, a Zinc Finger Protein, Regulates Cancer Stemness

      • Hiroaki Taniguchi, Kohzoh Imai
      Pages 3-13

    3. Computational Methods for Analysis of the DNA-Binding Preferences of Cys2His2 Zinc-Finger Proteins

      • Berat Doğan, Hamed S. Najafabadi
      Pages 15-28

    4. Design and Application of 6mA-Specific Zinc-Finger Proteins for the Readout of DNA Methylation

      • Johannes A. H. Maier, Albert Jeltsch
      Pages 29-41

    5. Enhanced Manipulation of Human Mitochondrial DNA Heteroplasmy In Vitro Using Tunable mtZFN Technology

      • Payam A. Gammage, Michal Minczuk
      Pages 43-56

    6. Engineering RNA-Binding Proteins by Modular Assembly of RanBP2-Type Zinc Fingers

      • Simona De Franco, Mitchell R. O’Connell, Marylène Vandevenne
      Pages 57-74

    7. Design of a System for Monitoring Ubiquitination Activities of E2 Enzymes Using Engineered RING Finger Proteins

      • Kazuhide Miyamoto, Kazuki Saito
      Pages 75-87

    8. Directed Evolution of Targeted Recombinases for Genome Engineering

      • Shannon J. Sirk
      Pages 89-102

    9. Optical Recording of Cellular Zinc Dynamics with Zinc-Finger-Based Biosensors

      • Dylan H. Fudge, Ray Black, Yan Qin
      Pages 103-112

    10. Delivery of Superoxide Dismutase Using Cys2-His2 Zinc-Finger Proteins

      • Jia Liu, Jiangmei Li, Jie Li, Lianhui Zhu, Shaojie Wang, Xuan Wei et al.
      Pages 113-123

    11. Genome Editing of MSCs as a Platform for Cell Therapy

      • Krissanapong Manotham, Supreecha Chattong
      Pages 125-138

  3. Methods for the Evaluation and Prevention of ZFN Cytotoxicity

    1. Front Matter

      Pages 139-139
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    2. Integrated Multimodal Evaluation of Genotoxicity in ZFN-Modified Primary Human Cells

      • Jaichandran Sivalingam, Dimitar Kenanov, Wai Har Ng, Sze Sing Lee, Toan Thang Phan, Sebastian Maurer-Stroh et al.
      Pages 141-164

    3. Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain

      • Maui Nishio, Ayana Yamagishi, Kaori Tsukakoshi, Yoshio Kato, Chikashi Nakamura, Kazunori Ikebukuro
      Pages 165-174

    4. An Improved Genome Engineering Method Using Surrogate Reporter-Coupled Suicidal ZFNs

      • Jiani Xing, Cunfang Zhang, Kun Xu, Linyong Hu, Ling Wang, Tingting Zhang et al.
      Pages 175-183

  4. Delivery Methods of ZFPs

    1. Front Matter

      Pages 185-185
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    2. Non-transgenic Approach to Deliver ZFNs in Seeds for Targeted Genome Engineering

      • Zoe Hilioti
      Pages 187-199

    3. Gene Editing in Channel Catfish via Double Electroporation of Zinc-Finger Nucleases

      • Rex A. Dunham, Ahmed Elaswad, Zhenkui Qin
      Pages 201-214

    4. Delivery of mtZFNs into Early Mouse Embryos

      • Beverly J. McCann, Andy Cox, Payam A. Gammage, James B. Stewart, Magdalena Zernicka-Goetz, Michal Minczuk
      Pages 215-228
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Keywords

About this book

This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.

Editors and Affiliations

  • Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China

    Jia Liu

Bibliographic Information

  • Book Title: Zinc Finger Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Jia Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8799-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8798-6Published: 28 August 2018

  • Softcover ISBN: 978-1-4939-9392-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8799-3Published: 28 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 276

  • Number of Illustrations: 15 b/w illustrations, 44 illustrations in colour

  • Topics: Human Genetics

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