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Electrophoretic Separation of Proteins

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1855)

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Table of contents (45 protocols)

  1. Front Matter

    Pages i-xv
  2. Introduction to Protein Electrophoresis

    • Pothur R. Srinivas
    Pages 23-29
  3. Cellulose Acetate Electrophoresis of Hemoglobin

    • Ramesh Kumar, Wilbert A. Derbigny
    Pages 81-85
  4. Native Polyacrylamide Gels

    • Claudia Arndt, Stefanie Koristka, Anja Feldmann, Michael Bachmann
    Pages 87-91
  5. Isoelectric Focusing on Non-Denaturing Flatbed Gels

    • Biji T. Kurien, R. Hal Scofield
    Pages 93-100
  6. Determination of Protein Molecular Weights on SDS-PAGE

    • Hiroyuki Matsumoto, Hisao Haniu, Naoka Komori
    Pages 101-105
  7. Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE

    • Hiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, Naoka Komori
    Pages 107-113
  8. Cationic Electrophoresis

    • Engelbert Buxbaum
    Pages 115-124
  9. Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients

    • Bre’Ana Byrd, Huyen Tran
    Pages 125-129
  10. SARCOSYL—PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins

    • Christian Reichel, Günter Gmeiner, Philipp Reihlen, Mario Thevis, Wilhelm Schänzer
    Pages 131-149
  11. Tricine-SDS-PAGE

    • Syed R. Haider, Helen J. Reid, Barry L. Sharp
    Pages 151-160
  12. Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis

    • Marta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, Jean M. H. van den Elsen
    Pages 161-175
  13. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis

    • Henghang Tsai, Hon-Chiu Eastwood Leung
    Pages 211-227

About this book

This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field.


Editors and Affiliations

  • Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    Biji T. Kurien, R. Hal Scofield

Bibliographic Information

  • Book Title: Electrophoretic Separation of Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Biji T. Kurien, R. Hal Scofield

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8793-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8792-4Published: 14 November 2018

  • eBook ISBN: 978-1-4939-8793-1Published: 13 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 523

  • Number of Illustrations: 51 b/w illustrations, 61 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access