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Electrophoretic Separation of Proteins

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Biji T. Kurien

    1. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. R. Hal Scofield

    1. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1855)

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Table of contents (45 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. How It All Began: A Personal History of Gel Electrophoresis

    • Oliver Smithies
    Pages 1-21

  3. Introduction to Protein Electrophoresis

    • Pothur R. Srinivas
    Pages 23-29

  4. Measuring Protein Concentration with Absorbance, Lowry, Bradford Coomassie Blue, or the Smith Bicinchoninic Acid Assay Before Electrophoresis

    • J. P. Dean Goldring
    Pages 31-39

  5. Concentrating Proteins by Salt, Polyethylene Glycol, Solvent, SDS Precipitation, Three-Phase Partitioning, Dialysis, Centrifugation, Ultrafiltration, Lyophilization, Affinity Chromatography, Immunoprecipitation or Increased Temperature for Protein Isolation, Drug Interaction, and Proteomic and Peptidomic Evaluation

    • J. P. Dean Goldring
    Pages 41-59

  6. Lysis Buffer Choices Are Key Considerations to Ensure Effective Sample Solubilization for Protein Electrophoresis

    • Ewa I. Miskiewicz, Daniel J. MacPhee
    Pages 61-72

  7. The Cydex Blue Assay: A One-Step Protein Assay for Samples Prior to SDS Electrophoresis

    • Thierry Rabilloud
    Pages 73-80

  8. Cellulose Acetate Electrophoresis of Hemoglobin

    • Ramesh Kumar, Wilbert A. Derbigny
    Pages 81-85

  9. Native Polyacrylamide Gels

    • Claudia Arndt, Stefanie Koristka, Anja Feldmann, Michael Bachmann
    Pages 87-91

  10. Isoelectric Focusing on Non-Denaturing Flatbed Gels

    • Biji T. Kurien, R. Hal Scofield
    Pages 93-100

  11. Determination of Protein Molecular Weights on SDS-PAGE

    • Hiroyuki Matsumoto, Hisao Haniu, Naoka Komori
    Pages 101-105

  12. Two-Dimensional Gel Electrophoresis by Glass Tube-Based IEF and SDS-PAGE

    • Hiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, Naoka Komori
    Pages 107-113

  13. Cationic Electrophoresis

    • Engelbert Buxbaum
    Pages 115-124

  14. Two-Dimensional Gel Electrophoresis with Immobilized pH Gradients

    • Bre’Ana Byrd, Huyen Tran
    Pages 125-129

  15. SARCOSYL—PAGE: Optimized Protocols for the Separation and Immunological Detection of PEGylated Proteins

    • Christian Reichel, Günter Gmeiner, Philipp Reihlen, Mario Thevis, Wilhelm Schänzer
    Pages 131-149

  16. Tricine-SDS-PAGE

    • Syed R. Haider, Helen J. Reid, Barry L. Sharp
    Pages 151-160

  17. Analysis of Protein Glycation Using Phenylboronate Acrylamide Gel Electrophoresis

    • Marta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, Jean M. H. van den Elsen
    Pages 161-175

  18. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies

    • Gyorgy Csako
    Pages 177-201

  19. Electrophoretic Separation of Very Large Molecular Weight Proteins in SDS Agarose

    • Marion L. Greaser, Chad M. Warren
    Pages 203-210

  20. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis

    • Henghang Tsai, Hon-Chiu Eastwood Leung
    Pages 211-227
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Keywords

About this book

This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field.


Editors and Affiliations

  • Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, USA

    Biji T. Kurien, R. Hal Scofield

Bibliographic Information

  • Book Title: Electrophoretic Separation of Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Biji T. Kurien, R. Hal Scofield

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8793-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8792-4Published: 14 November 2018

  • eBook ISBN: 978-1-4939-8793-1Published: 13 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 523

  • Number of Illustrations: 51 b/w illustrations, 61 illustrations in colour

  • Topics: Protein Science

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