Methods in Molecular Biology

Xenopus

Methods and Protocols

Editors: Vleminckx, Kris (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8784-9
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8783-2
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About this book

This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis

    McNamara, Sean (et al.)

    Pages 1-17

  • Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos

    Wlizla, Marcin (et al.)

    Pages 19-32

  • Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering

    Naert, Thomas (et al.)

    Pages 33-54

  • Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology

    Van Nieuwenhuysen, Tom (et al.)

    Pages 55-65

  • Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis

    Naert, Thomas (et al.)

    Pages 67-82

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8784-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8783-2
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Xenopus
Book Subtitle
Methods and Protocols
Editors
  • Kris Vleminckx
Series Title
Methods in Molecular Biology
Series Volume
1865
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8784-9
DOI
10.1007/978-1-4939-8784-9
Hardcover ISBN
978-1-4939-8783-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 302
Number of Illustrations
11 b/w illustrations, 57 illustrations in colour
Topics