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Transgenic Plants

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Sandeep Kumar

    1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Pierluigi Barone

    1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Michelle Smith

    1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the use of transgenic plant technology
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1864)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Plant Transformation and Vector Construction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Repurposing Macromolecule Delivery Tools for Plant Genetic Modification in the Era of Precision Genome Engineering

      • Qiudeng Que, Mary-Dell M. Chilton, Sivamani Elumalai, Heng Zhong, Shujie Dong, Liang Shi
      Pages 3-18

    3. The Use of an Automated Platform to Assemble Multigenic Constructs for Plant Transformation

      • David G. J. Mann, Scott A. Bevan, Anthony J. Harvey, Rachelle A. Leffert-Sorenson
      Pages 19-35

    4. Ensifer-Mediated Transformation (EMT) of Rice (Monocot) and Oilseed Rape (Dicot)

      • Dheeraj Singh Rathore, Evelyn Zuniga-Soto, Ewen Mullins
      Pages 37-48

    5. Setaria viridis as a Model Plant for Functional Genomic Studies in C4 Crops

      • Polyana Kelly Martins, Bárbara Andrade Dias Brito da Cunha, Adilson Kenji Kobayshi, Hugo Bruno Correa Molinari
      Pages 49-65

    6. Transient Transformation Using Particle Bombardment for Gene Expression Analysis

      • Andika Gunadi, Eric A. Dean, John J. Finer
      Pages 67-79

    7. Maize Transformation Using the Morphogenic Genes Baby Boom and Wuschel2

      • Todd Jones, Keith Lowe, George Hoerster, Ajith Anand, Emily Wu, Ning Wang et al.
      Pages 81-93

    8. Efficient and Fast Production of Transgenic Rice Plants by Agrobacterium-Mediated Transformation

      • Chuanyin Wu, Yi Sui
      Pages 95-103

    9. Protocol for Agrobacterium-Mediated Transformation and Transgenic Plant Production of Switchgrass

      • QiuXia Chen, Guo-Qing Song
      Pages 105-115

    10. Biolistic Transformation of Wheat

      • Bin Tian, Mónica Navia-Urrutia, Yueying Chen, Jordan Brungardt, Harold N. Trick
      Pages 117-130

    11. Mesophyll Protoplasts and PEG-Mediated Transfections: Transient Assays and Generation of Stable Transgenic Canola Plants

      • Sareena Sahab, Matthew J. Hayden, John Mason, German Spangenberg
      Pages 131-152

    12. A Unified Agrobacterium-Mediated Transformation Protocol for Alfalfa (Medicago sativa L.) and Medicago truncatula

      • Qingzhen Jiang, Chunxiang Fu, Zeng-Yu Wang
      Pages 153-163

    13. Poplar Transformation

      • Tobias Bruegmann, Olaf Polak, Khira Deecke, Julia Nietsch, Matthias Fladung
      Pages 165-177

    14. The Genetic Transformation of Sweet Orange (Citrus sinensis L. Osbeck) for Enhanced Resistance to Citrus Canker

      • Lorena Noelia Sendin, María Paula Filippone
      Pages 179-190

    15. Genetic Modification of Grapevine Embryogenic Cultures

      • S. A. Dhekney, S. K. Sessions, M. Brungart-Rosenberg, C. Claflin, Z. T. Li, D. J. Gray
      Pages 191-201

    16. Agrobacterium-Mediated Transformation of Solanum tuberosum L., Potato

      • Myron A. Bruce, Jessica L. Shoup Rupp
      Pages 203-223

    17. Agrobacterium tumefaciens-Mediated Transformation of Tomato

      • Joyce Van Eck, Patricia Keen, Michelle Tjahjadi
      Pages 225-234

  3. Genome Editing

    1. Front Matter

      Pages 235-235
      Download chapter PDF

    2. DNA Break Repair in Plants and Its Application for Genome Engineering

      • Carla Schmidt, Michael Pacher, Holger Puchta
      Pages 237-266
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Keywords

About this book

This book provides thorough coverage of transgenic plants with methods on plant transformation, biotechnological application of transgenic plants, and future developments. Chapters are grouped into sections focusing on transformation model and crop plants, genome engineering, and transgenic event characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Transgenic Plants: Methods and Protocols aims to broaden the utility for readers, provide additional references for further understanding, and present the technology’s potential for solving some of our most urgent global challenges in food security.

Editors and Affiliations

  • Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, USA

    Sandeep Kumar, Pierluigi Barone, Michelle Smith

Bibliographic Information

  • Book Title: Transgenic Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Sandeep Kumar, Pierluigi Barone, Michelle Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8778-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8777-1Published: 11 November 2018

  • eBook ISBN: 978-1-4939-8778-8Published: 10 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 444

  • Number of Illustrations: 4 b/w illustrations, 73 illustrations in colour

  • Topics: Plant Sciences

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