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Methods in Molecular Biology

X-Chromosome Inactivation

Methods and Protocols

Editors: Sado, Takashi (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-8766-5
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About this book

This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs

    Pages 1-18

    Monfort, Asun (et al.)

  • Unbiased Genetic Screen to Identify Factors Involved in X-Chromosome Inactivation Using a Pooled Bar-Coded shRNA Library

    Pages 19-36

    Moindrot, Benoit (et al.)

  • ChIRP-MS: RNA-Directed Proteomic Discovery

    Pages 37-45

    Chu, Ci (et al.)

  • Manipulation of Xist Imprinting in Mouse Preimplantation Embryos

    Pages 47-53

    Fukuda, Atsushi (et al.)

  • Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status

    Pages 55-65

    Inoue, Kimiko (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-8766-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $139.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
X-Chromosome Inactivation
Book Subtitle
Methods and Protocols
Editors
  • Takashi Sado
Series Title
Methods in Molecular Biology
Series Volume
1861
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8766-5
DOI
10.1007/978-1-4939-8766-5
Hardcover ISBN
978-1-4939-8765-8
Softcover ISBN
978-1-4939-9386-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 251
Number of Illustrations
10 b/w illustrations, 43 illustrations in colour
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