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Methods in Molecular Biology

SNAREs

Methods and Protocols

Editors: Fratti, Rutilio (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8760-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8759-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week). Pre-ordered printed titles are excluded from promotions.
  • Due: October 28, 2019
  • ISBN 978-1-4939-9385-7
  • Free shipping for individuals worldwide
About this book

This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Molecular Dynamics Simulations of the SNARE Complex

    Pages 3-13

    Bykhovskaia, Maria

  • Mesoscale Computational Modeling of Protein-Membrane Interactions Based on Continuum Mean-Field Theory

    Pages 15-31

    Khelashvili, George

  • EPR Lineshape Analysis to Investigate the SNARE Folding Intermediates

    Pages 33-51

    Khounlo, Ryan (et al.)

  • Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion

    Pages 53-69

    Kong, Byoungjae (et al.)

  • SNAREpin Assembly: Kinetic and Thermodynamic Approaches

    Pages 71-93

    Li, Feng (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8760-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8759-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week). Pre-ordered printed titles are excluded from promotions.
  • Due: October 28, 2019
  • ISBN 978-1-4939-9385-7
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
SNAREs
Book Subtitle
Methods and Protocols
Editors
  • Rutilio Fratti
Series Title
Methods in Molecular Biology
Series Volume
1860
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8760-3
DOI
10.1007/978-1-4939-8760-3
Hardcover ISBN
978-1-4939-8759-7
Softcover ISBN
978-1-4939-9385-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 407
Number of Illustrations
8 b/w illustrations, 66 illustrations in colour
Topics