Methods in Molecular Biology

Protein Gel Detection and Imaging

Methods and Protocols

Editors: Kurien, Biji T., Scofield, Hal (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8745-0
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8744-3
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.

Table of contents (33 chapters)

  • Protein Stains and Applications

    Sundaram, Pazhani

    Pages 1-14

  • The Roles of Acetic Acid and Methanol During Fixing and Staining Proteins in an SDS–Polyacrylamide Electrophoresis Gel

    Goldring, J. P. Dean

    Pages 15-18

  • Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of Protein Bands During SDS-PAGE

    Kumar, Gaurav

    Pages 19-25

  • Coomassie Brilliant Blue Staining of Polyacrylamide Gels

    Arndt, Claudia (et al.)

    Pages 27-30

  • A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Using Coomassie Blue

    Dong, Wei-hua (et al.)

    Pages 31-35

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8745-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8744-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Gel Detection and Imaging
Book Subtitle
Methods and Protocols
Editors
  • Biji T. Kurien
  • Hal Scofield
Series Title
Methods in Molecular Biology
Series Volume
1853
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8745-0
DOI
10.1007/978-1-4939-8745-0
Hardcover ISBN
978-1-4939-8744-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 289
Number of Illustrations and Tables
4 b/w illustrations, 35 illustrations in colour
Topics