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Microbial Carotenoids

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1852)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
  2. Advancement of Biotechnology by Genetic Modifications

    • Arnold L. Demain, Sergio Sánchez
    Pages 1-43
  3. Carotenoids Production: A Healthy and Profitable Industry

    • Carlos Barreiro, José-Luis Barredo
    Pages 45-55
  4. Carotenoids: From Plants to Food and Feed Industries

    • Panagiota Langi, Sotirios Kiokias, Theodoros Varzakas, Charalampos Proestos
    Pages 57-71
  5. Preparative Recovery of Carotenoids from Microalgal Biomass

    • María del Carmen Cerón García, Cynthia Victoria González López, José María Fernández Sevilla, Emilio Molina Grima
    Pages 107-115
  6. Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae

    • Yixi Su, Zhiqian Yi, Snædís Huld Björnsdóttir, Sigurdur Brynjolfsson, Weiqi Fu
    Pages 117-126
  7. Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-Producing Bacteria

    • Dalal Asker, Tarek S. Awad, Teruhiko Beppu, Kenji Ueda
    Pages 143-170
  8. Screening, Isolation, and Identification of Zeaxanthin-Producing Bacteria

    • Dalal Asker, Tarek S. Awad, Teruhiko Beppu, Kenji Ueda
    Pages 193-209
  9. Synthesis of Carotenoids of Industrial Interest in the Photosynthetic Bacterium Rhodopseudomonas palustris : Bioengineering and Growth Conditions

    • Eric Giraud, Laure Hannibal, Clémence Chaintreuil, Joël Fardoux, André Verméglio
    Pages 211-220
  10. Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides

    • Francisco E. Nicolás, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Sergio López-García, Eusebio Navarro, Santiago Torres-Martínez et al.
    Pages 221-237
  11. Expression Vectors and Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides

    • Enrique A. Iturriaga, María Isabel Alvarez, Arturo P. Eslava, Tamás Papp
    Pages 239-256
  12. Lycopene Production by Mated Fermentation of Blakeslea trispora

    • Sonia Martínez-Cámara, Sara Rubio, Hannah del Río, Marta Rodríguez-Sáiz, José-Luis Barredo
    Pages 257-268
  13. HPLC Analysis of Carotenoids in Neurosporaxanthin-Producing Fungi

    • Dámaso Hornero-Méndez, M. Carmen Limón, Javier Avalos
    Pages 269-281
  14. Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous

    • Carlos García-Estrada, Katarina Kosalková, Isabel-Clara Sánchez-Orejas
    Pages 283-295
  15. Isolation and Selection of New Astaxanthin-Producing Strains of Phaffia rhodozyma

    • Diego Libkind, Martín Moliné, Fernando Colabella
    Pages 297-310
  16. Engineering Pichia pastoris for the Production of Carotenoids

    • Patricia Veiga-Crespo, José Miguel Araya-Garay, Tomás G. Villa
    Pages 311-326

About this book

In this volume, expert researchers in the field detail the most up-to-date methods commonly used to study and produce carotenoids. These include methods on the manipulation and metabolic engineering of carotenoid producing microalgae and bacteria, including Corynebacterium glutamicum, Rhodopseudomonas palustris and radio-tolerant bacteria; in addition to fungi, as the beta-carotene producing Blakeslea trispora and Mucor circinelloides or the lycopene producing Blakeslea trispora; and the heterobasidiomycetous yeast producing xanthophylls Xanthophyllomyces dendrorhous (Phaffia rhodozyma) and the engineered yeast Pichia pastoris. Additionally, three overview chapters on the advancement of Biotechnology and carotenoid production are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Microbial Carotenoids: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms, aiming to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, León, Spain

    Carlos Barreiro

  • Department of Biotechnology, Crystal Pharma, A Subsidiary of Albany, Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

Bibliographic Information

  • Book Title: Microbial Carotenoids

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos Barreiro, José-Luis Barredo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8742-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8741-2Published: 14 August 2018

  • Softcover ISBN: 978-1-4939-9380-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8742-9Published: 14 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 343

  • Number of Illustrations: 37 b/w illustrations, 21 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access