Methods in Molecular Biology

Clathrin-Mediated Endocytosis

Methods and Protocols

Editors: Swan, Laura (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This book presents a methodological introduction on clathrin-mediated endocytosis. Chapters guide readers through a number of model systems, purifications, reconstitutions, analysis techniques and systemic alterations of membrane function, which has been pivotal in understanding the role of clathrin and is panoply of interactors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Clathrin-Mediated Endoytsis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Purification of Clathrin-Coated Vesicles from Adult Rat Brain

    Pages 1-11

    Giovedì, Silvia

  • Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation

    Pages 13-22

    Messa, Mirko

  • Probing Endocytosis During the Cell Cycle with Minimal Experimental Perturbation

    Pages 23-35

    Santos, António J. M. (et al.)

  • Assaying the Contribution of Membrane Tension to Clathrin-Mediated Endocytosis

    Pages 37-50

    Boulant, Steeve

  • Identifying Small-Molecule Inhibitors of the Clathrin Terminal Domain

    Pages 51-64

    Haucke, Volker (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-4939-8719-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8717-7
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-9374-1
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Clathrin-Mediated Endocytosis
Book Subtitle
Methods and Protocols
Editors
  • Laura Swan
Series Title
Methods in Molecular Biology
Series Volume
1847
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8719-1
DOI
10.1007/978-1-4939-8719-1
Hardcover ISBN
978-1-4939-8717-7
Softcover ISBN
978-1-4939-9374-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 253
Number of Illustrations
13 b/w illustrations, 38 illustrations in colour
Topics