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Methods in Molecular Biology

The Ubiquitin Proteasome System

Methods and Protocols

Editors: Mayor, Thibault, Kleiger, Gary (Eds.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8706-1
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8705-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: October 6, 2019
  • ISBN 978-1-4939-9371-0
  • Free shipping for individuals worldwide
About this book

“This volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest advances to study the formation of poly-ubiquitin chains as well as their linkage types; the binding partners of proteins in the UPS; methods for structure determination by x-ray crystallography, cryo electron microscopy and SAXS; screening assays to select for degrons or modulators of E3s and DUBs; proteomics approaches in the ubiquitin field and methods to study 26S proteasome function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Thorough and authoritative, The Ubiquitin Proteasome System: Methods and Protocols is a valuable resource for both experienced and novice scientists who are interested in expanding their knowledge in this field.


Table of contents (25 chapters)

Table of contents (25 chapters)
  • Characterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms

    Pages 3-17

    Reiter, Katherine H. (et al.)

  • Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays

    Pages 19-31

    Buetow, Lori (et al.)

  • Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins

    Pages 33-38

    Qiu, Jiazhang (et al.)

  • Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for Their Ubiquitin Ligase Partners

    Pages 39-58

    Hill, Spencer (et al.)

  • Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity

    Pages 59-70

    Morgan, Michael T. (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8706-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8705-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: October 6, 2019
  • ISBN 978-1-4939-9371-0
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
The Ubiquitin Proteasome System
Book Subtitle
Methods and Protocols
Editors
  • Thibault Mayor
  • Gary Kleiger
Series Title
Methods in Molecular Biology
Series Volume
1844
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8706-1
DOI
10.1007/978-1-4939-8706-1
Hardcover ISBN
978-1-4939-8705-4
Softcover ISBN
978-1-4939-9371-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 413
Number of Illustrations
29 b/w illustrations, 39 illustrations in colour
Topics