The Ubiquitin Proteasome System
Methods and Protocols
Editors: Mayor, Thibault, Kleiger, Gary (Eds.)
Free Preview- Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts
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- About this book
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“This volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest advances to study the formation of poly-ubiquitin chains as well as their linkage types; the binding partners of proteins in the UPS; methods for structure determination by x-ray crystallography, cryo electron microscopy and SAXS; screening assays to select for degrons or modulators of E3s and DUBs; proteomics approaches in the ubiquitin field and methods to study 26S proteasome function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and authoritative, The Ubiquitin Proteasome System: Methods and Protocols is a valuable resource for both experienced and novice scientists who are interested in expanding their knowledge in this field.
- Table of contents (25 chapters)
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Characterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms
Pages 3-17
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Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays
Pages 19-31
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Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins
Pages 33-38
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Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for Their Ubiquitin Ligase Partners
Pages 39-58
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Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity
Pages 59-70
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Table of contents (25 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- The Ubiquitin Proteasome System
- Book Subtitle
- Methods and Protocols
- Editors
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- Thibault Mayor
- Gary Kleiger
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1844
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8706-1
- DOI
- 10.1007/978-1-4939-8706-1
- Hardcover ISBN
- 978-1-4939-8705-4
- Softcover ISBN
- 978-1-4939-9371-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 413
- Number of Illustrations
- 29 b/w illustrations, 39 illustrations in colour
- Topics
