Methods in Molecular Biology

Microbial Proteomics

Methods and Protocols

Editors: Becher, Dörte (Ed.)

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  • Includes cutting-edge methods and protocols for the study of microbial proteomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-8695-8
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About this book

This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond.
Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Filter-Aided Sample Preparation for Proteome Analysis

    Wiśniewski, Jacek R.

    Pages 3-10

  • Protein Enrichment from Highly Dilute Samples with StrataClean

    Bonn, Florian (et al.)

    Pages 11-18

  • Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins

    Sievers, Susanne

    Pages 21-33

  • Enrichment of Cell Surface-Associated Proteins in Gram-Positive Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis

    Bonn, Florian (et al.)

    Pages 35-43

  • Preparation of Bacterial Magnetosomes for Proteome Analysis

    Raschdorf, Oliver (et al.)

    Pages 45-57

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8695-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8693-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Microbial Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Dörte Becher
Series Title
Methods in Molecular Biology
Series Volume
1841
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8695-8
DOI
10.1007/978-1-4939-8695-8
Hardcover ISBN
978-1-4939-8693-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 344
Number of Illustrations
24 b/w illustrations, 26 illustrations in colour
Topics