- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.
- Table of contents (15 chapters)
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Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
Pages 1-9
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Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype
Pages 11-28
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Preparation of Vibrio cholerae Samples for RNA-seq Analysis
Pages 29-38
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Random Transposon Mutagenesis of Vibrio cholerae
Pages 39-44
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Metabolomics of Vibrio cholerae
Pages 45-51
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Table of contents (15 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Vibrio Cholerae
- Book Subtitle
- Methods and Protocols
- Editors
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- Aleksandra E. Sikora
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1839
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-8685-9
- DOI
- 10.1007/978-1-4939-8685-9
- Hardcover ISBN
- 978-1-4939-8684-2
- Softcover ISBN
- 978-1-4939-9365-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 180
- Number of Illustrations
- 8 b/w illustrations, 19 illustrations in colour
- Topics
