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  • © 2018

The Human Virome

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1838)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. General Experimental Methods

    1. Front Matter

      Pages 1-1
    2. Protocol for Generating Infectious RNA Viromes from Complex Biological Samples

      • Sonia Monteil-Bouchard, Sarah Temmam, Christelle Desnues
      Pages 25-36
  3. Gastrointestinal Tract

    1. Front Matter

      Pages 47-47
    2. Extraction and Purification of Viruses from Fecal Samples for Metagenome and Morphology Analyses

      • Josué L. Castro-Mejía, Ling Deng, Finn K. Vogensen, Alejandro Reyes, Dennis S. Nielsen
      Pages 49-57
    3. Virome Sequencing of Stool Samples

      • Lenka Kramná, Ondřej Cinek
      Pages 59-83
    4. NetoVIR: Modular Approach to Customize Sample Preparation Procedures for Viral Metagenomics

      • Nádia Conceição-Neto, Kwe Claude Yinda, Marc Van Ranst, Jelle Matthijnssens
      Pages 85-95
  4. Respiratory Tract

    1. Front Matter

      Pages 109-109
    2. Targeted Sequencing of Respiratory Viruses in Clinical Specimens for Pathogen Identification and Genome-Wide Analysis

      • Yu Yang, Shannon D. Walls, Stephen M. Gross, Gary P. Schroth, Richard G. Jarman, Jun Hang
      Pages 125-140
  5. Oral Cavity

    1. Front Matter

      Pages 141-141
  6. Blood–Plasma

    1. Front Matter

      Pages 163-163
    2. A Method for Isolation of the Virome from Plasma Samples

      • Pilar Domingo-Calap, Juan Vicente Bou
      Pages 165-171
  7. Urinary Tract

    1. Front Matter

      Pages 189-189

About this book

This book details the most comprehensive, up-to –date, and cutting-edge protocols used in wet and dry labs to investigate the viral communities harbored within and on the human body. Chapters guide readers through methods on collection, isolation, identification and computational/statistical analysis, and body niches to cover those methodological issues inherent to the human tissues and organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, The Human Virome: Methods and Protocols aims to facilitate researchers with their daily work in the field of the research on the human virome.

Editors and Affiliations

  • Institute for Integrative Systems Biology, University of València, València, Spain

    Andrés Moya

  • Foundation for the Promotion of Health and Biomedical Research of the Valencian Community (FISABIO), València, Spain

    Vicente Pérez Brocal

Bibliographic Information

  • Book Title: The Human Virome

  • Book Subtitle: Methods and Protocols

  • Editors: Andrés Moya, Vicente Pérez Brocal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8682-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8681-1Published: 21 August 2018

  • Softcover ISBN: 978-1-4939-9364-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8682-8Published: 20 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 274

  • Number of Illustrations: 8 b/w illustrations, 34 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access