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Influenza Virus

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols for the study of the influenza virus
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1836)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvi
  2. Understanding Influenza

    • Edward C. Hutchinson, Yohei Yamauchi
    Pages 1-21
  3. Clinical Diagnosis of Influenza

    • Yoshinori Ito
    Pages 23-31
  4. Influenza A Virus Genetic Tools: From Clinical Sample to Molecular Clone

    • Stéphanie Anchisi, Ana Rita Gonçalves, Béryl Mazel-Sanchez, Samuel Cordey, Mirco Schmolke
    Pages 33-58
  5. Propagation and Titration of Influenza Viruses

    • Umut Karakus, Michel Crameri, Caroline Lanz, Emilio Yángüez
    Pages 59-88
  6. Purification and Proteomics of Influenza Virions

    • Edward C. Hutchinson, Monika Stegmann
    Pages 89-120
  7. Deciphering Virus Entry with Fluorescently Labeled Viral Particles

    • Anja B. Hoffmann, Magalie Mazelier, Psylvia Léger, Pierre-Yves Lozach
    Pages 159-183
  8. Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape

    • Wen-Chi Su, Michael M. C. Lai
    Pages 185-194
  9. Correlative Light and Electron Microscopy of Influenza Virus Entry and Budding

    • Lorna Hodgson, Paul Verkade, Yohei Yamauchi
    Pages 237-260
  10. Metal-Tagging Transmission Electron Microscopy and Immunogold Labeling on Tokuyasu Cryosections to Image Influenza A Virus Ribonucleoprotein Transport and Packaging

    • Martin Sachse, Isabel Fernández de Castro, Guillaume Fournier, Nadia Naffakh, Cristina Risco
    Pages 281-301
  11. Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy

    • Amar R. Bhagwat, Valerie Le Sage, Seema S. Lakdawala
    Pages 303-327
  12. Purification of Unanchored Polyubiquitin Chains from Influenza Virions

    • Yasuyuki Miyake, Patrick Matthias, Yohei Yamauchi
    Pages 329-342
  13. Assays to Measure the Activity of Influenza Virus Polymerase

    • Aartjan J. W. te Velthuis, Jason S. Long, Wendy S. Barclay
    Pages 343-374
  14. In Vitro Models to Study Influenza Virus and Staphylococcus aureus Super-Infection on a Molecular Level

    • Christin Bruchhagen, Andre van Krüchten, Carolin Klemm, Stephan Ludwig, Christina Ehrhardt
    Pages 375-386
  15. Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus

    • Hannah M. Creager, Terrence M. Tumpey, Taronna R. Maines, Jessica A. Belser
    Pages 387-399

About this book

This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging, antigenic evolution, immunology to mathematical modelling. Reviews cover general influenza, clinical trials, both sides of the gain-of-function debate, and computational modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Influenza Virus: Methods and Protocols aims to motivate experienced researchers and newcomers in the field and improve our overall understanding of influenza.

Editors and Affiliations

  • School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    Yohei Yamauchi

Bibliographic Information

  • Book Title: Influenza Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Yohei Yamauchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8678-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8677-4Published: 28 August 2018

  • Softcover ISBN: 978-1-4939-9363-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8678-1Published: 27 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 663

  • Number of Illustrations: 23 b/w illustrations, 160 illustrations in colour

  • Topics: Infectious Diseases

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access