Skip to main content
SpringerLink
Log in

Retinal Degeneration

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Bernhard H. F. Weber

    1. Institute of Human Genetics, University of Regensburg, Regensburg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Thomas Langmann

    1. Department of Ophthalmology, University of Cologne, Cologne, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1834)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Molecular Genetics and Tools

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Identification and Analysis of Genes Associated with Inherited Retinal Diseases

      • Mubeen Khan, Zeinab Fadaie, Stéphanie S. Cornelis, Frans P. M. Cremers, Susanne Roosing
      Pages 3-27

    3. Conduct and Quality Control of Differential Gene Expression Analysis Using High-Throughput Transcriptome Sequencing (RNASeq)

      • Felix Grassmann
      Pages 29-43

    4. Testing for Known Retinal Degeneration Mutants in Mouse Strains

      • Khalid Rashid, Katharina Dannhausen, Thomas Langmann
      Pages 45-58

    5. CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo

      • Daniela Benati, Valeria Marigo, Alessandra Recchia
      Pages 59-74

    6. Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis

      • Yuchen Lin, Sarah Irmscher, Christine Skerka
      Pages 75-83

  3. Cell/Tissue Culture Models

    1. Front Matter

      Pages 85-85
      Download chapter PDF

    2. Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells

      • Caroline Brandl
      Pages 87-94

    3. Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture

      • Francesca Mazzoni, Yingyu Mao, Silvia C. Finnemann
      Pages 95-108

    4. Porcine RPE/Choroidal Explant Cultures

      • Alexa Klettner, Yoko Miura
      Pages 109-118

    5. The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells

      • Manuela Völkner, Thomas Kurth, Mike O. Karl
      Pages 119-141

    6. Cell Death Analysis in Retinal Cultures

      • Sarah L. Roche, Ana M. Ruiz-Lopez, Thomas G. Cotter
      Pages 143-152

    7. Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-Derived Macrophages in Retinal Disease

      • Nancy J. Reyes, Rose Mathew, Daniel R. Saban
      Pages 153-164

  4. Animal Models

    1. Front Matter

      Pages 165-165
      Download chapter PDF

    2. Light Damage Models of Retinal Degeneration

      • Christian Grimm, Charlotte E. Remé
      Pages 167-178

    3. Induction and Readout of Oxygen-Induced Retinopathy

      • Raffael Liegl, Claudia Priglinger, Andreas Ohlmann
      Pages 179-191

    4. Generation and Analysis of Xenopus laevis Models of Retinal Degeneration Using CRISPR/Cas9

      • Joanna M. Feehan, Paloma Stanar, Beatrice M. Tam, Colette Chiu, Orson L. Moritz
      Pages 193-207

    5. Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases

      • Holger Dill, Utz Fischer
      Pages 209-219

    6. Drosophila melanogaster: A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa

      • Malte Lehmann, Elisabeth Knust, Sarita Hebbar
      Pages 221-249
Back to top

Keywords

About this book

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.

Editors and Affiliations

  • Institute of Human Genetics, University of Regensburg, Regensburg, Germany

    Bernhard H. F. Weber

  • Department of Ophthalmology, University of Cologne, Cologne, Germany

    Thomas Langmann

Bibliographic Information

  • Book Title: Retinal Degeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Bernhard H. F. Weber, Thomas Langmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8669-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8668-2Published: 16 October 2018

  • Softcover ISBN: 978-1-4939-9360-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8669-9Published: 15 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 417

  • Number of Illustrations: 52 b/w illustrations, 56 illustrations in colour

  • Topics: Molecular Medicine

Publish with us

Policies and ethics

Back to top

Search

Navigation