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Methods in Molecular Biology

Retinal Degeneration

Methods and Protocols

Editors: Weber, Bernhard, Langmann, Thomas (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-8669-9
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About this book

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Identification and Analysis of Genes Associated with Inherited Retinal Diseases

    Pages 3-27

    Khan, Mubeen (et al.)

  • Conduct and Quality Control of Differential Gene Expression Analysis Using High-Throughput Transcriptome Sequencing (RNASeq)

    Pages 29-43

    Grassmann, Felix

  • Testing for Known Retinal Degeneration Mutants in Mouse Strains

    Pages 45-58

    Rashid, Khalid (et al.)

  • CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo

    Pages 59-74

    Benati, Daniela (et al.)

  • Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis

    Pages 75-83

    Lin, Yuchen (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-8669-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8668-2
  • with online files
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9360-4
  • Free shipping for individuals worldwide. COVID-19 shipping restrictions apply.
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Retinal Degeneration
Book Subtitle
Methods and Protocols
Editors
  • Bernhard Weber
  • Thomas Langmann
Series Title
Methods in Molecular Biology
Series Volume
1834
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8669-9
DOI
10.1007/978-1-4939-8669-9
Hardcover ISBN
978-1-4939-8668-2
Softcover ISBN
978-1-4939-9360-4
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 417
Number of Illustrations
52 b/w illustrations, 56 illustrations in colour
Topics

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