Methods in Molecular Biology

Histone Variants

Methods and Protocols

Editors: Orsi, Guillermo, Almouzni, Genevieve (Eds.)

Free Preview
  • Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
Show all benefits

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-8663-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $219.99
price for USA in USD
Softcover $219.99
price for USA in USD
About this book

This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information. Its four sections cover experimental approaches to probe the biochemistry of histone variants and variant nucleosomes; their dynamics throughout the cell cycle and at specific genomic locations; their functional impact on chromosome organization and genome stability; and their importance in development, disease and evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, illustrations on the expected results, and tips on troubleshooting and avoiding known pitfalls. This anthology brings together contributions from many of the most highly recognized investigators in this community.

Cutting-edge and thorough, Histone Variants: Methods and Protocols is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field.


Table of contents (19 chapters)

Table of contents (19 chapters)
  • Methods for Preparing Nucleosomes Containing Histone Variants

    Pages 3-20

    Kujirai, Tomoya (et al.)

  • Characterization of Posttranslational Modifications on Histone Variants

    Pages 21-49

    Saavedra, Francisco (et al.)

  • Purification of Histone Variant-Interacting Chaperone Complexes

    Pages 51-60

    Tagami, Hideaki

  • Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics

    Pages 61-74

    Völker-Albert, Moritz Carl (et al.)

  • Histone Native Chromatin Immunoprecipitation

    Pages 77-104

    Alonso, Alicia (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-8663-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $219.99
price for USA in USD
Softcover $219.99
price for USA in USD
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Histone Variants
Book Subtitle
Methods and Protocols
Editors
  • Guillermo Orsi
  • Genevieve Almouzni
Series Title
Methods in Molecular Biology
Series Volume
1832
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8663-7
DOI
10.1007/978-1-4939-8663-7
Hardcover ISBN
978-1-4939-8662-0
Softcover ISBN
978-1-4939-9358-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 359
Number of Illustrations
19 b/w illustrations, 43 illustrations in colour
Topics