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Antibody Engineering

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Damien Nevoltris

    1. Immunology Division, Garvan Institute of Medical Research, Darlinghurst, Australia

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  2. Patrick Chames

    1. Institut Paoli-Calmettes, CRCM, Aix Marseille University, CNRS, INSERM, Marseille, France

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  • Includes cutting-edge techniques for the study of antibody engineering
  • Provides step-by-step detail essential for reproducible results
  • Contains updated key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1827)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xvi
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  2. In Silico Methods

    1. Front Matter

      Pages 1-1
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    2. Antibody Design and Humanization via In Silico Modeling

      • Vinodh B. Kurella, Reddy Gali
      Pages 3-14

    3. Antibody Affinity Maturation by Computational Design

      • Daisuke Kuroda, Kouhei Tsumoto
      Pages 15-34

    4. Use of IMGT® Databases and Tools for Antibody Engineering and Humanization

      • Marie-Paule Lefranc, François Ehrenmann, Sofia Kossida, Véronique Giudicelli, Patrice Duroux
      Pages 35-69

  3. Generation of Diversity

    1. Front Matter

      Pages 71-71
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    2. Construction of Human Naïve Antibody Gene Libraries

      • Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, Katia Scotlandi
      Pages 73-91

    3. Construction of Synthetic Antibody Libraries

      • Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau
      Pages 93-108

    4. Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments

      • Doreen Könning, Steffen Hinz, Julius Grzeschik, Christian Schröter, Simon Krah, Stefan Zielonka et al.
      Pages 109-127

    5. Display Technologies for Generation of Ig Single Variable Domains

      • Vladimir Bobkov, Bas van der Woning, Hans de Haard
      Pages 129-144

    6. A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries

      • Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir et al.
      Pages 145-161

  4. Selections of Lead Candidate

    1. Front Matter

      Pages 163-163
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    2. Phage Display and Selections on Purified Antigens

      • Magali Colazet, Patrick Chames
      Pages 165-178

    3. Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display

      • Martina L. Jones, Stephen M. Mahler, Sumukh Kumble
      Pages 179-195

    4. Selection of Antibody Fragments Against Structured DNA by Phage Display

      • Mahdi Zeraati, Marcel E. Dinger, Daniel Christ
      Pages 197-209

    5. Selection of Antibody Fragments by Yeast Display

      • Nathalie Scholler
      Pages 211-233

    6. Rapid Selection of High-Affinity Antibody scFv Fragments Using Ribosome Display

      • Birgit Dreier, Andreas Plückthun
      Pages 235-268

    7. In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display

      • Naoto Nemoto, Shigefumi Kumachi, Hidenao Arai
      Pages 269-285

    8. Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood

      • Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ et al.
      Pages 287-309

  5. Production of Recombinant Antibodies

    1. Front Matter

      Pages 311-311
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Keywords

About this book

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques.  The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Editors and Affiliations

  • Immunology Division, Garvan Institute of Medical Research, Darlinghurst, Australia

    Damien Nevoltris

  • Institut Paoli-Calmettes, CRCM, Aix Marseille University, CNRS, INSERM, Marseille, France

    Patrick Chames

Bibliographic Information

  • Book Title: Antibody Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Damien Nevoltris, Patrick Chames

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8648-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8647-7Published: 09 September 2018

  • Softcover ISBN: 978-1-4939-9353-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8648-4Published: 08 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVI, 543

  • Number of Illustrations: 33 b/w illustrations, 73 illustrations in colour

  • Topics: Immunology

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