Methods in Molecular Biology

Mouse Oocyte Development

Methods and Protocols

Editors: Verlhac, Marie-Hélène, Terret, Marie-Emilie (Eds.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.

Reviews

“Each chapter presents not only detailed and reproducible methods (troubleshooting included) but also nice images and instructive figures providing non-verbal information necessary to better understand how to deal with this ‘fantastic little laboratory of molecular biology’ that is the oocyte.” (Manuela Monti, European Journal of Histochemistry, Vol. 62 (04), October, 2018)

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Growth In Vitro of Granulosa Cell–Oocyte Complexes of the Mouse

    Pages 1-11

    El-Hayek, Stephany (et al.)

  • In Vitro Culture of Mouse Oocytes for Meiotic Maturation

    Pages 13-21

    Borsuk, Ewa (et al.)

  • In Vitro Maturation and Culture of Human Oocytes

    Pages 23-30

    Gruhn, Jennifer R. (et al.)

  • Pronuclear Transfer in Human Oocytes

    Pages 31-36

    Hyslop, Louise

  • In Vitro Fertilization of Mouse Oocyte

    Pages 37-42

    Chaigne, Agathe

Buy this book

eBook n/a
  • ISBN 978-1-4939-8603-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-4939-8602-6
  • Free shipping for individuals worldwide
Softcover n/a
  • ISBN 978-1-4939-9340-6
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Mouse Oocyte Development
Book Subtitle
Methods and Protocols
Editors
  • Marie-Hélène Verlhac
  • Marie-Emilie Terret
Series Title
Methods in Molecular Biology
Series Volume
1818
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8603-3
DOI
10.1007/978-1-4939-8603-3
Hardcover ISBN
978-1-4939-8602-6
Softcover ISBN
978-1-4939-9340-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 174
Number of Illustrations
13 b/w illustrations, 30 illustrations in colour
Topics