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Methods in Molecular Biology

ADP-ribosylation and NAD+ Utilizing Enzymes

Methods and Protocols

Editors: Chang, Paul (Ed.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8588-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8587-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: August 25, 2019
  • ISBN 978-1-4939-9335-2
  • Free shipping for individuals worldwide
About this book

This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.


Table of contents (28 chapters)

Table of contents (28 chapters)
  • Vitamin B3 in Health and Disease: Toward the Second Century of Discovery

    Pages 3-8

    Jacobson, Myron K. (et al.)

  • Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently Tagged Macrodomains

    Pages 11-24

    Smith, Rebecca (et al.)

  • In Vitro Techniques for ADP-Ribosylated Substrate Identification

    Pages 25-40

    Grimaldi, Giovanna (et al.)

  • Assessment of Intracellular Auto-Modification Levels of ARTD10 Using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8

    Pages 41-63

    Bütepage, Mareike (et al.)

  • Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR-Binding Analysis

    Pages 65-73

    Wang, Zhizhi (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8588-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8587-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: August 25, 2019
  • ISBN 978-1-4939-9335-2
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
ADP-ribosylation and NAD+ Utilizing Enzymes
Book Subtitle
Methods and Protocols
Editors
  • Paul Chang
Series Title
Methods in Molecular Biology
Series Volume
1813
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8588-3
DOI
10.1007/978-1-4939-8588-3
Hardcover ISBN
978-1-4939-8587-6
Softcover ISBN
978-1-4939-9335-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 417
Number of Illustrations
44 b/w illustrations, 51 illustrations in colour
Topics