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ADP-ribosylation and NAD+ Utilizing Enzymes

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Paul Chang

    1. Department of Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1813)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Vitamin B3 in Health and Disease: Toward the Second Century of Discovery

      • Myron K. Jacobson, Elaine L. Jacobson
      Pages 3-8

  3. ADP-Ribose Binding Domains as Tools

    1. Front Matter

      Pages 9-9
      Download chapter PDF

    2. Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently Tagged Macrodomains

      • Rebecca Smith, Gyula Timinszky
      Pages 11-24

    3. In Vitro Techniques for ADP-Ribosylated Substrate Identification

      • Giovanna Grimaldi, Giuliana Catara, Carmen Valente, Daniela Corda
      Pages 25-40

    4. Assessment of Intracellular Auto-Modification Levels of ARTD10 Using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8

      • Mareike Bütepage, Sarah Krieg, Laura Eckei, Jinyu Li, Giulia Rossetti, Patricia Verheugd et al.
      Pages 41-63

    5. Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR-Binding Analysis

      • Zhizhi Wang, Wenqing Xu
      Pages 65-73

  4. Enzymatic Assays of NAD+ Utilizing Enzymes

    1. Front Matter

      Pages 75-75
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    2. Assays for NAD+-Dependent Reactions and NAD+ Metabolites

      • Michael B. Schultz, Yuancheng Lu, Nady Braidy, David A. Sinclair
      Pages 77-90

    3. Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro

      • Ken Y. Lin, Dan Huang, W. Lee Kraus
      Pages 91-108

    4. Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity

      • David Hutin, Giulia Grimaldi, Jason Matthews
      Pages 109-124

    5. Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events

      • Anna-Lena Kolb, Duen-Wei Hsu, Ana B. A. Wallis, Seiji Ura, Alina Rakhimova, Catherine J. Pears et al.
      Pages 125-148

    6. Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases

      • Linda A. Stevens, Joel Moss
      Pages 149-165

    7. Monitoring Expression and Enzyme Activity of Ecto-ARTCs

      • Stephan Menzel, Sahil Adriouch, Peter Bannas, Friedrich Haag, Friedrich Koch-Nolte
      Pages 167-186

    8. ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins

      • Masato Mashimo, Joel Moss
      Pages 187-204

    9. Mono-ADP-Ribosylhydrolase Assays

      • Jeannette Abplanalp, Ann-Katrin Hopp, Michael O. Hottiger
      Pages 205-213

    10. Hydrolysis of ADP-Ribosylation by Macrodomains

      • Melanija Posavec Marjanovic´, Gytis Jankevicius, Ivan Ahel
      Pages 215-223

    11. HPLC-Based Enzyme Assays for Sirtuins

      • Jun Young Hong, Xiaoyu Zhang, Hening Lin
      Pages 225-234

  5. Small Molecule Screening Assays of NAD+ Utilizing Enzymes

    1. Front Matter

      Pages 235-235
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Keywords

About this book

This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.



Editors and Affiliations

  • Department of Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, USA

    Paul Chang

Bibliographic Information

  • Book Title: ADP-ribosylation and NAD+ Utilizing Enzymes

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Chang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8588-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8587-6Published: 11 August 2018

  • Softcover ISBN: 978-1-4939-9335-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8588-3Published: 10 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 417

  • Number of Illustrations: 44 b/w illustrations, 51 illustrations in colour

  • Topics: Biochemistry, general

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