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Platelets and Megakaryocytes

Volume 4, Advanced Protocols and Perspectives

  • Book
  • © 2018

Overview

Editors:
  1. Jonathan M. Gibbins

    1. Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Martyn Mahaut-Smith

    1. Department of Molecular and Cell Biology, University of Leicester, Leicester, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1812)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Immobilization of Nonactivated Unfixed Platelets for Real-Time Single-Cell Analysis

    • Alexander P. Bye, Zeki Ilkan, Amanda J. Unsworth, Chris I. Jones
    Pages 1-11

  3. Imaging Platelets and Megakaryocytes by High-Resolution Laser Fluorescence Microscopy

    • Fred G. Pluthero, Walter H. A. Kahr
    Pages 13-31

  4. Single-Molecule Localization and Structured Illumination Microscopy of Platelet Proteins

    • Natalie S. Poulter, Abdullah O. Khan, Chiara Pallini, Steven G. Thomas
    Pages 33-54

  5. Electron Tomography and Correlative Approaches in Platelet Studies

    • Kasia B. Engberts, Cor Seinen, Willie J. C. Geerts, Harry F. G. Heijnen
    Pages 55-79

  6. Screening and High-Throughput Platelet Assays

    • Alexander P. Bye, Amanda J. Unsworth, Jonathan M. Gibbins
    Pages 81-94

  7. High-Throughput Signaling Profiling in Blood Platelets by Multiplexed Phosphoflow Cytometry

    • Benjamin E. J. Spurgeon, Khalid M. Naseem
    Pages 95-111

  8. Precise Quantification of Platelet Proteins and Their Phosphorylation States

    • Francoise Mazet, Michael J. Fry
    Pages 113-125

  9. The Study of Platelet Receptors Using Artificial Lipid Bilayers

    • Michael L. Dustin, Alice Y. Pollitt
    Pages 127-137

  10. Three-Dimensional Culture in a Methylcellulose-Based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation

    • Alicia Aguilar, Julie Boscher, Fabien Pertuy, Christian Gachet, Catherine Léon
    Pages 139-153

  11. Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor-Driven Forward Programming

    • Thomas Moreau, Amanda L. Evans, Cedric J. G. Ghevaert
    Pages 155-176

  12. Three-Dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production

    • Christian A. Di Buduo, Vittorio Abbonante, Lorenzo Tozzi, David L. Kaplan, Alessandra Balduini
    Pages 177-193

  13. Fluorescence Approaches to Image and Quantify the Demarcation Membrane System in Living Megakaryocytes

    • Sangar Osman, Daniel Dalmay, Martyn Mahaut-Smith
    Pages 195-215

  14. High-Resolution 3D Imaging of Megakaryocytes Using Focused Ion Beam-Scanning Electron Microscopy

    • Anita Eckly, Jean-Yves Rinckel, Fabienne Proamer, Christian Gachet
    Pages 217-231

  15. Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-Sheet Fluorescence Microscopy

    • Maximilian G. Gorelashvili, Katrin G. Heinze, David Stegner
    Pages 233-253

  16. Mathematical Techniques for Understanding Platelet Regulation and the Development of New Pharmacological Approaches

    • Joanna L. Dunster, Mikhail A. Panteleev, Jonathan M. Gibbins, Anastacia N. Sveshnikova
    Pages 255-279

  17. Back Matter

    Pages 281-290
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Keywords

About this book

The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives.  Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new methods for platelet production in vitro, and systems biology approaches. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting or  avoiding known pitfalls.

Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 4, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.

Editors and Affiliations

  • Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK

    Jonathan M. Gibbins

  • Department of Molecular and Cell Biology, University of Leicester, Leicester, UK

    Martyn Mahaut-Smith

Bibliographic Information

  • Book Title: Platelets and Megakaryocytes

  • Book Subtitle: Volume 4, Advanced Protocols and Perspectives

  • Editors: Jonathan M. Gibbins, Martyn Mahaut-Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8585-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8584-5Published: 01 September 2018

  • Softcover ISBN: 978-1-4939-9334-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8585-2Published: 31 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 290

  • Number of Illustrations: 20 b/w illustrations, 52 illustrations in colour

  • Topics: Cell Biology

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