Methods in Molecular Biology

Handbook of ELISPOT

Methods and Protocols

Editors: Kalyuzhny, Alexander E. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8567-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8566-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.”

Table of contents (20 chapters)

  • Challenges in Developing Protein Secretion Assays at a Single-Cell Level

    Shirasaki, Yoshitaka (et al.)

    Pages 1-7

  • Mastering the Computational Challenges of Elispot Plate Evaluation

    Janetzki, Sylvia

    Pages 9-30

  • Essential Controls for ELISpot Assay

    Hagen, Jodi (et al.)

    Pages 31-41

  • Automatic Search of Spots and Color Classification in ELISPOT Assay

    Zadorozhny, Sergey S. (et al.)

    Pages 43-50

  • Four Color ImmunoSpot® Assays for Identification of Effector T-Cell Lineages

    Hanson, Jodi (et al.)

    Pages 51-62

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-8567-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8566-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Handbook of ELISPOT
Book Subtitle
Methods and Protocols
Editors
  • Alexander E. Kalyuzhny
Series Title
Methods in Molecular Biology
Series Volume
1808
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-8567-8
DOI
10.1007/978-1-4939-8567-8
Hardcover ISBN
978-1-4939-8566-1
Series ISSN
1064-3745
Edition Number
3
Number of Pages
X, 251
Number of Illustrations and Tables
6 b/w illustrations, 68 illustrations in colour
Topics