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Progranulin

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Andrew Bateman

    1. Department of Medicine, McGill University MUHC, Montreal, Canada

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  2. Hugh P.J. Bennett

    1. Department of Medicine, McGill University MUHC, Montreal, Canada

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    You can also search for this editor in PubMed   Google Scholar

  3. Siu Tim Cheung

    1. Department of Surgery, The Chinese University of Hong Kong, Hong Kong, China

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1806)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. A Brief Overview of Progranulin in Health and Disease

      • Andrew Bateman, Siu Tim Cheung, Hugh P. J. Bennett
      Pages 3-15

  3. Analytical and In Vitro Methods for the Study of Progranulin Biology

    1. Front Matter

      Pages 17-17
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    2. Chromatographic Methods for the Purification of Granulin Peptides

      • Andrew Bateman, Babykumari P. Chitramuthu, Hugh P. J. Bennett
      Pages 19-34

    3. Methods for Expression and Purification of Biologically Active Recombinant Progranulin

      • Chun Yu Zhao, Andrew Bateman
      Pages 35-49

    4. Large-Scale Generation of Recombinant Granulin Peptides in E. coli

      • Dmitri Tolkatchev
      Pages 51-64

    5. Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging

      • Dmitri Tolkatchev
      Pages 65-80

    6. Data Mining: Applying the AD&FTD Mutation Database to Progranulin

      • Marc Cruts, Christine Van Broeckhoven
      Pages 81-92

  4. Methods to Investigate the Role of Progranulin in Cancer

    1. Front Matter

      Pages 93-93
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    2. Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases

      • Ginette Serrero, David Hicks
      Pages 95-105

    3. Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker

      • Ginette Serrero, David Hicks
      Pages 107-120

    4. Analysis of Progranulin-Mediated Akt and MAPK Activation

      • Shi-Qiong Xu, Simone Buraschi, Ryuta Tanimoto, Manuela Stefanello, Antonino Belfiore, Renato V. Iozzo et al.
      Pages 121-130

    5. Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models

      • Chi Wai Yip, Phyllis F. Y. Cheung, Nicholas C. L. Wong, Sze Wai Fung, Siu Tim Cheung
      Pages 131-144

    6. Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features

      • Phyllis F. Y. Cheung, Siu Tim Cheung
      Pages 145-153

    7. Methods to Study the Role of Progranulin in the Tumor Microenvironment

      • Moshe Elkabets, Samuel Brook
      Pages 155-176

  5. Animal Models to Study the Role of Progranulin in Health and Disease

    1. Front Matter

      Pages 177-177
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    2. Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo Using Caenorhabditis elegans

      • James Julian Doyle, J. Alex Parker
      Pages 179-191

    3. The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response

      • Tsung-Yuan Hsu, Victoria J. Butler, Aimee W. Kao
      Pages 193-206

    4. Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function

      • Babykumari P. Chitramuthu, Hugh P. J. Bennett
      Pages 207-231
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Keywords

About this book

This volume explores experimental strategies to study progranulin as a regulatory protein in biological and disease processes. The chapters in this book are divided into four parts: Part One is an introduction to the topic; Part Two looks at analytical and in vitro methods to study progranulin biology; Part Three discusses the role of progranulin in cancer; and Part Four describes the process of using animal models to study progranulin in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Progranulin: Methods and Protocols is a valuable resource for experienced researchers who want to expand their studies of this field, and newcomers who want to begin including progranulin into their work.

Editors and Affiliations

  • Department of Medicine, McGill University MUHC, Montreal, Canada

    Andrew Bateman, Hugh P.J. Bennett

  • Department of Surgery, The Chinese University of Hong Kong, Hong Kong, China

    Siu Tim Cheung

Bibliographic Information

  • Book Title: Progranulin

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew Bateman, Hugh P.J. Bennett, Siu Tim Cheung

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8559-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8557-9Published: 28 June 2018

  • Softcover ISBN: 978-1-4939-9325-3Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8559-3Published: 28 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 363

  • Number of Illustrations: 23 b/w illustrations, 56 illustrations in colour

  • Topics: Protein Science

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