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Plant Chemical Genomics

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Friedrich Fauser

    1. Department of Molecular Biology, Princeton University, Princeton, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Martin Jonikas

    1. Department of Molecular Biology, Princeton University, Princeton, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1795)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Multi-Parametric Screening in Arabidopsis thaliana Seedlings

    • Long Nguyen, Andrzej Drozdzecki, Vera Goossens, Bert De Rybel, Tom Beeckman, Dominique Audenaert
    Pages 1-7

  3. Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress

    • Susan Fozard, Brian G. Forde
    Pages 9-25

  4. Method for Ultrarapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen

    • Roman Chuprov-Netochin, Elena Marusich, Yaroslav Neskorodov, Yana Mishutkina, Polina Volynchuk, Yan Ivanenkov et al.
    Pages 27-37

  5. A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death Using Suspension Cell Culture of Arabidopsis thaliana

    • Yoshiteru Noutoshi, Ken Shirasu
    Pages 39-47

  6. High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression

    • Vivek Halder, Erich Kombrink
    Pages 49-63

  7. Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana Using Chemical Genetics

    • Clémence Bonnot, Laurent Nussaume, Thierry Desnos
    Pages 65-84

  8. Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls Using BY-2 Cells

    • Emiko Okubo-Kurihara, Minami Matsui
    Pages 85-92

  9. Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes

    • Mitsuhiro Kimura, Sachiko Isobe
    Pages 93-99

  10. High-Throughput In Vitro Screening for Inhibitors of Cereal α-Glucosidase

    • Michael D. Rugen, Martin Rejzek, Henrik Naested, Birte Svensson, Robert A. Field
    Pages 101-115

  11. Chemical Screening for Strigolactone Receptor Antagonists Using Arabidopsis thaliana

    • Duncan Holbrook-Smith, Peter McCourt
    Pages 117-126

  12. Chemical Control of ABA Receptors to Enable Plant Protection Against Water Stress

    • Masanori Okamoto, Sean R. Cutler
    Pages 127-141

  13. Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening

    • Yajin Ye, Yang Zhao
    Pages 143-148

  14. Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside That Controls the Leaf Closure of Samanea saman

    • Yousuke Takaoka, Minoru Ueda
    Pages 149-158

  15. Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule

    • Yoichi Takakusagi, Kaori Takakusagi, Fumio Sugawara, Kengo Sakaguchi
    Pages 159-172

  16. Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants Using PlantiSMASH

    • Satria A. Kautsar, Hernando G. Suarez Duran, Marnix H. Medema
    Pages 173-188

  17. Chemical Genomics Translatability from Unicellular to Multicellular Models

    • Cecilia Rodriguez-Furlán, Carlos Rubilar-Hernández, Lorena Norambuena
    Pages 189-201

  18. Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii

    • Brittany Jack, Prachee Avasthi
    Pages 203-221

  19. FnCpf1-Mediated Targeted Mutagenesis in Plants

    • Akira Endo, Seiichi Toki
    Pages 223-239

  20. Erratum to: Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii

    • Brittany Jack, Prachee Avasthi
    Pages E1-E1
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Keywords

About this book

This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.


Editors and Affiliations

  • Department of Molecular Biology, Princeton University, Princeton, USA

    Friedrich Fauser, Martin Jonikas

Bibliographic Information

  • Book Title: Plant Chemical Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Friedrich Fauser, Martin Jonikas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7874-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7873-1Published: 30 May 2018

  • Softcover ISBN: 978-1-4939-9311-6Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7874-8Published: 30 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 242

  • Number of Illustrations: 13 b/w illustrations, 47 illustrations in colour

  • Topics: Plant Sciences

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