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Methods in Molecular Biology

Two-Hybrid Systems

Methods and Protocols

Editors: Oñate-Sánchez, Luis (Ed.)

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  • Includes cutting-edge methods and protocols for the study of two-hybrid systems
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7871-7
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Hardcover $279.99
price for USA in USD
  • ISBN 978-1-4939-7870-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $279.99
price for USA in USD
  • ISBN 978-1-4939-9310-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab. 
Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • Protein Interactomics by Two-Hybrid Methods

    Pages 1-14

    Choi, Soon Gang (et al.)

  • Making the Right Choice: Critical Parameters of the Y2H Systems

    Pages 17-28

    Mehla, Jitender (et al.)

  • Identification of Protein–Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening

    Pages 29-48

    Lathouwers, Thomas (et al.)

  • Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System

    Pages 49-60

    Asseck, Lisa Yasmin (et al.)

  • The Ras Recruitment System (RRS) for the Identification and Characterization of Protein–Protein Interactions

    Pages 61-73

    Aronheim, Ami

Buy this book

eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7871-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $279.99
price for USA in USD
  • ISBN 978-1-4939-7870-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $279.99
price for USA in USD
  • ISBN 978-1-4939-9310-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Two-Hybrid Systems
Book Subtitle
Methods and Protocols
Editors
  • Luis Oñate-Sánchez
Series Title
Methods in Molecular Biology
Series Volume
1794
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-7871-7
DOI
10.1007/978-1-4939-7871-7
Hardcover ISBN
978-1-4939-7870-0
Softcover ISBN
978-1-4939-9310-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 355
Number of Illustrations
8 b/w illustrations, 50 illustrations in colour
Topics