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Methods in Molecular Biology

Epitope Mapping Protocols

Editors: Rockberg, Johan, Nilvebrant, Johan (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7841-0
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7839-7
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Softcover $199.99
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  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: May 15, 2019
  • ISBN 978-1-4939-9301-7
  • Free shipping for individuals worldwide
About this book

This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • An Introduction to Epitope Mapping

    Pages 1-10

    Nilvebrant, Johan (et al.)

  • Epitope Mapping of Antibody-Antigen Interactions with X-Ray Crystallography

    Pages 13-27

    Toride King, Moeko (et al.)

  • Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations

    Pages 29-51

    Simonelli, Luca (et al.)

  • Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors

    Pages 53-63

    Peiris, Diluka (et al.)

  • Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically Encoded Photo-Activatable Cross-Linkers

    Pages 65-75

    Huber, Thomas (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7841-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7839-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.99
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: May 15, 2019
  • ISBN 978-1-4939-9301-7
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Epitope Mapping Protocols
Editors
  • Johan Rockberg
  • Johan Nilvebrant
Series Title
Methods in Molecular Biology
Series Volume
1785
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-7841-0
DOI
10.1007/978-1-4939-7841-0
Hardcover ISBN
978-1-4939-7839-7
Softcover ISBN
978-1-4939-9301-7
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XI, 284
Number of Illustrations
11 b/w illustrations, 38 illustrations in colour
Topics