Macrophages

1. Front Matter

   Pages i-xii

   PDF

2. Culture of Human Monocyte-Derived Macrophages

   • Aoife Kelly, Aleksander M. Grabiec, Mark A. Travis

   Pages 1-11

3. A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages

   • Chandrayana Mukherjee, Christine Hale, Subhankar Mukhopadhyay

   Pages 13-28Open Access

4. Growing Murine Bone Marrow-Derived Macrophages

   • Anaïs Assouvie, Lisa P. Daley-Bauer, Germain Rousselet

   Pages 29-33

5. Immortalization of Murine Bone Marrow-Derived Macrophages

   • Dominic De Nardo, Dhan V. Kalvakolanu, Eicke Latz

   Pages 35-49

6. Isolation and Identification of Murine Serous Cavity Macrophages

   • Calum C. Bain, Stephen J. Jenkins

   Pages 51-67

7. Isolation and Identification of Interstitial Macrophages from the Lungs Using Different Digestion Enzymes and Staining Strategies

   • Shaikh M. Atif, Sophie L. Gibbings, Claudia V. Jakubzick

   Pages 69-76

8. Isolation and Phenotyping of Adult Mouse Microglial Cells

   • Kathleen Grabert, Barry W. McColl

   Pages 77-86Open Access

9. Isolation and Phenotyping of Bone Marrow Macrophages

   • Marion Chalot

   Pages 87-92

10. Isolation and Phenotyping of Intestinal Macrophages

    • Vanessa Petit

    Pages 93-98

11. Phenotyping Tumor-Associated Macrophages

    • R. Ian Cumming, Yen-Rei A. Yu

    Pages 99-109

12. Activating Murine Macrophages In Vitro

    • Elizabeth Dalby

    Pages 111-117

13. Polarizing Macrophages In Vitro

    • Xuan Huang, Yong Li, Mingui Fu, Hong-Bo Xin

    Pages 119-126

14. Viral Replication Assay in Bone Marrow-Derived Macrophages

    • Linda Roback, Lisa P. Daley-Bauer

    Pages 127-134

15. Macrophage Bactericidal Assays

    • Mourad Aribi

    Pages 135-149

16. Quantitative Phagocytosis Assays in Primary and Cultured Macrophages

    • Fernando Montaño, Sergio Grinstein, Roni Levin

    Pages 151-163

17. Observing Frustrated Phagocytosis and Phagosome Formation and Closure Using Total Internal Reflection Fluorescence Microscopy (TIRFM)

    • Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, Florence Niedergang

    Pages 165-175

18. Chromatin Immunoprecipitation in Macrophages

    • Germain Rousselet

    Pages 177-186

19. Transfecting Macrophages

    • Andrea-Anneliese Keller, Marten B. Maeß, Michael Schnoor, Berith Scheiding, Stefan Lorkowski

    Pages 187-195

20. In Vitro Migration Assays

    • Lewis Taylor, Carlota Recio, David R. Greaves, Asif J. Iqbal

    Pages 197-214

This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.


The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.

• phenotyping
• chlodronate
• Chromatin immunoprecipitation
• inflammasome
• Influenza

• CEA/DRF/Jacob/iRCM/LRTS, INSERM U967, Fontenay aux Roses, France

  Germain Rousselet

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