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Planarian Regeneration

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Jochen C. Rink

    1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1774)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Planarian Diversity and Phylogeny

    • Ronald Sluys, Marta Riutort
    Pages 1-56

  3. Stem Cells, Patterning and Regeneration in Planarians: Self-Organization at the Organismal Scale

    • Jochen C. Rink
    Pages 57-172

  4. The Ecology of Freshwater Planarians

    • Miquel Vila-Farré, Jochen C. Rink
    Pages 173-205

  5. Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology

    • Sarah A. Elliott, Alejandro Sánchez Alvarado
    Pages 207-239

  6. Culturing Planarians in the Laboratory

    • M. Shane Merryman, Alejandro Sánchez Alvarado, James C. Jenkin
    Pages 241-258

  7. Total RNA Isolation from Planarian Tissues

    • Shang-Yun Liu, Jochen C. Rink
    Pages 259-265

  8. Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians

    • Markus A. Grohme, Miquel Vila-Farré, Jochen C. Rink
    Pages 267-275

  9. Planarian High Molecular Weight DNA Isolation by Spooling

    • Shasha Zhang, Alejandro Sánchez Alvarado
    Pages 277-284

  10. Basic Histological Techniques for Planarians

    • Leigh Winsor, Ronald Sluys
    Pages 285-351

  11. Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians

    • David J. Forsthoefel, Kelly G. Ross, Phillip A. Newmark, Ricardo M. Zayas
    Pages 353-366

  12. Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections

    • Teresa Adell, Sara Barberán, Miquel Sureda-Gómez, María Almuedo-Castillo, Nidia de Sousa, Francesc Cebrià
    Pages 367-378

  13. Whole-Mount In Situ Hybridization of Planarians

    • Ryan S. King, Phillip A. Newmark
    Pages 379-392

  14. RNA In Situ Hybridization on Planarian Paraffin Sections

    • Jordi Solana
    Pages 393-404

  15. General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods

    • José María Martín-Durán
    Pages 405-421

  16. Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians

    • Li-Chun Cheng, Alejandro Sánchez Alvarado
    Pages 423-434

  17. Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL

    • Brad Stubenhaus, Jason Pellettieri
    Pages 435-444

  18. Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria

    • Carolyn E. Adler, Alejandro Sánchez Alvarado
    Pages 445-454

  19. RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA

    • Norito Shibata, Kiyokazu Agata
    Pages 455-466

  20. A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells

    • Tetsutaro Hayashi, Kiyokazu Agata
    Pages 467-478
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Keywords

About this book

This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.

Editors and Affiliations

  • Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

    Jochen C. Rink

About the editor

Jochen Rink, PhD
Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden, Germany
Tel: +49-(0)351-210-2435
Email: rink@mpi-cbg.de 

Bibliographic Information

  • Book Title: Planarian Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Jochen C. Rink

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7802-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7800-7Published: 19 June 2018

  • Softcover ISBN: 978-1-4939-9289-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7802-1Published: 18 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 573

  • Number of Illustrations: 36 b/w illustrations, 56 illustrations in colour

  • Topics: Cell Biology

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