- Includes cutting-edge methods and protocols for the study of synthetic biology
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
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This detailed book explores a few of the plethora of techniques and applications associated with the unique branch of science known as synthetic biology. Chemists, biologists, and engineers engaged in this multidisciplinary field of study will be guided in the creation and regulation of gene circuits, manipulation of biochemical pathways, genome editing and modification, creating genome language and computing, as well as molecular assembly. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, plus troubleshooting tips and explanations of how to avoid pitfalls, all written by experts who have published their work in peer-reviewed journals.
Authoritative and practical, Synthetic Biology: Methods and Protocols provides key guidance and ideas for conducting your own synthetic biology projects.
- Table of contents (29 chapters)
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Re-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria
Pages 3-24
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Negative Regulation Gene Circuits for Efflux Pump Control
Pages 25-43
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Methods for Generating Microbial Cocultures that Grow in the Absence of Fixed Carbon or Nitrogen
Pages 45-60
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Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits
Pages 61-93
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Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast Starmerella bombicola
Pages 95-123
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Table of contents (29 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Synthetic Biology
- Book Subtitle
- Methods and Protocols
- Editors
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- Jeffrey Carl Braman
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1772
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-7795-6
- DOI
- 10.1007/978-1-4939-7795-6
- Hardcover ISBN
- 978-1-4939-7794-9
- Softcover ISBN
- 978-1-4939-9287-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 510
- Number of Illustrations
- 27 b/w illustrations, 82 illustrations in colour
- Topics
