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Cell-Based Microarrays

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1771)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. Next-Generation Live-Cell Microarray Technologies

      • Mario Rothbauer, Peter Ertl
      Pages 3-8
  3. Biointerface Engineering and Live-Cell Microarrays

    1. Front Matter

      Pages 9-9
    2. 3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell Patterning

      • Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos et al.
      Pages 27-40
    3. Single Cell Microarrays Fabricated by Microscale Plasma-Initiated Protein Patterning (μPIPP)

      • Anita Reiser, Matthias Lawrence Zorn, Alexandra Murschhauser, Joachim Oskar Rädler
      Pages 41-54
    4. Artificial Microniche Array with Spatially Structured Biochemical Cues

      • Xumei Gao, Celine Stoecklin, Yue Zhang, Zhe Weng, Richard De Mets, Gianluca Grenci et al.
      Pages 55-66
  4. Printing Techniques for Live-Cell Microarrays

    1. Front Matter

      Pages 67-67
    2. Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3D Microenvironments

      • Jorge F. Pascoal, Tiago G. Fernandes, Gregory J. Nierode, Maria Margarida Diogo, Jonathan S. Dordick, Joaquim M. S. Cabral
      Pages 69-81
    3. Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing

      • Julie Foncy, Aurore Estève, Amélie Degache, Camille Colin, Jean Christophe Cau, Laurent Malaquin et al.
      Pages 83-95
    4. Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabrication

      • Wenguang Yang, Haibo Yu, Yuechao Wang, Lianqing Liu
      Pages 97-105
    5. Inkjet Printing for Biomedical Applications

      • Christopher Chi Wai Tse, Patrick J. Smith
      Pages 107-117
  5. Live-Cell Microarray Application

    1. Front Matter

      Pages 119-119
    2. Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems

      • Rebecca Jonczyk, Thomas Scheper, Frank Stahl
      Pages 131-145
    3. Fabrication of Multielectrode Arrays for Neurobiology Applications

      • Mario Malerba, Hayder Amin, Gian N. Angotzi, Alessandro Maccione, Luca Berdondini
      Pages 147-157
  6. Microfluidic Live-Cell Arrays

    1. Front Matter

      Pages 159-159
    2. Rapid Prototyping of Thermoplastic Microfluidic Devices

      • Richard Novak, Carlos F. Ng, Donald E. Ingber
      Pages 161-170

About this book

This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays. Some of the topics covered are artificial micro-niche array with spatially structured biochemical cues; inkjet printing for biomedical application; fabrication of multi-electrode arrays for neurobiology applications; rapid prototyping of thermoplastic microfluidic devices; and oxygen-generating photocrosslinkable hydrogel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cell-Based Microarrays: Methods and Protocols is a valuable resource for anyone interested in expanding their knowledge and research of cell-based microarrays.

Editors and Affiliations

  • Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Peter Ertl, Mario Rothbauer

Bibliographic Information

  • Book Title: Cell-Based Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Peter Ertl, Mario Rothbauer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7792-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7791-8Published: 10 April 2018

  • Softcover ISBN: 978-1-4939-9286-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7792-5Published: 09 April 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 260

  • Number of Illustrations: 17 b/w illustrations, 66 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access