Methods in Molecular Biology

Protein Complex Assembly

Methods and Protocols

Editors: Marsh, Joseph (Ed.)

  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts 
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7759-8
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  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
  • ISBN 978-1-4939-7758-1
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About this book

This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (32 chapters)

  • Experimental Characterization of Protein Complex Structure, Dynamics, and Assembly

    Wells, Jonathan N. (et al.)

    Pages 3-27

  • High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly

    Henderson, Louie D. (et al.)

    Pages 29-44

  • Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy

    Gilmore, Brian L. (et al.)

    Pages 45-58

  • Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip

    Kaledhonkar, Sandip (et al.)

    Pages 59-71

  • Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy

    Ortega-Roldan, Jose Luis (et al.)

    Pages 73-85

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7759-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
  • ISBN 978-1-4939-7758-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Complex Assembly
Book Subtitle
Methods and Protocols
Editors
  • Joseph Marsh
Series Title
Methods in Molecular Biology
Series Volume
1764
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-7759-8
DOI
10.1007/978-1-4939-7759-8
Hardcover ISBN
978-1-4939-7758-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 506
Number of Illustrations and Tables
14 b/w illustrations, 79 illustrations in colour
Topics