- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
- Table of contents (32 chapters)
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Experimental Characterization of Protein Complex Structure, Dynamics, and Assembly
Pages 3-27
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High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly
Pages 29-44
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Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy
Pages 45-58
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Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip
Pages 59-71
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Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy
Pages 73-85
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Table of contents (32 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Protein Complex Assembly
- Book Subtitle
- Methods and Protocols
- Editors
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- Joseph Marsh
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1764
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-7759-8
- DOI
- 10.1007/978-1-4939-7759-8
- Hardcover ISBN
- 978-1-4939-7758-1
- Softcover ISBN
- 978-1-4939-9277-5
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 506
- Number of Illustrations
- 14 b/w illustrations, 79 illustrations in colour
- Topics
