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Methods in Molecular Biology

Reporter Gene Assays

Methods and Protocols

Editors: Damoiseaux, Robert, Hasson, Samuel (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7724-6
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About this book

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.  Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Genome-Edited Cell Lines for High-Throughput Screening

    Pages 1-17

    Dranchak, Patricia (et al.)

  • High-Throughput Firefly Luciferase Reporter Assays

    Pages 19-29

    Siebring-van Olst, Ellen (et al.)

  • Using the 2A Protein Coexpression System: Multicistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins

    Pages 31-48

    Luke, Garry A. (et al.)

  • Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter

    Pages 49-64

    Ramanathan, Chidambaram (et al.)

  • High-Throughput Screening Method to Identify Alternative Splicing Regulators

    Pages 65-74

    Stork, Cheryl (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7724-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-7722-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-9268-3
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Reporter Gene Assays
Book Subtitle
Methods and Protocols
Editors
  • Robert Damoiseaux
  • Samuel Hasson
Series Title
Methods in Molecular Biology
Series Volume
1755
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-7724-6
DOI
10.1007/978-1-4939-7724-6
Hardcover ISBN
978-1-4939-7722-2
Softcover ISBN
978-1-4939-9268-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 234
Number of Illustrations
11 b/w illustrations, 42 illustrations in colour
Topics

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