Cell Migration

1. Front Matter

   Pages i-xiv

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2. Random Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories

   • Irene Dang, Alexis Gautreau

   Pages 1-9

3. Directional Collective Migration in Wound Healing Assays

   • Nicolas Molinie, Alexis Gautreau

   Pages 11-19

4. An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition

   • Aleksandra S. Chikina, Antonina Y. Alexandrova

   Pages 21-27

5. An In Vitro System to Study the Epithelial–Mesenchymal Transition In Vitro

   • Natalya A. Gloushankova, Svetlana N. Rubtsova, Irina Y. Zhitnyak

   Pages 29-42

6. Detection of Migrasomes

   • Yang Chen, Ying Li, Liang Ma, Li Yu

   Pages 43-49

7. 3D Endothelial Cell Migration

   • Kathryn A. Jacobs, Julie Gavard

   Pages 51-58

8. Transmigration of Leukocytes Across Epithelial Monolayers

   • Penny E. Morton, Maddy Parsons

   Pages 59-70

9. Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay

   • Selma Maacha, Simon Saule

   Pages 71-77

10. Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multisample Format

    • Marleen Van Troys, Paola Masuzzo, Lynn Huyck, Karima Bakkali, Davy Waterschoot, Lennart Martens et al.

    Pages 79-117

11. Using Systems Microscopy to Understand the Emergence of Cell Migration from Cell Organization

    • Staffan Strömblad, John G. Lock

    Pages 119-134

12. Neuronal Precursor Migration in Ex Vivo Brain Slice Culture

    • Fu-Sheng Chou, Pei-Shan Wang

    Pages 135-143

13. In Vitro Models to Analyze the Migration of MGE-Derived Interneurons

    • Claire Leclech, Christine Métin

    Pages 145-161

14. Cell Migration in Tissues: Explant Culture and Live Imaging

    • Ralitza Staneva, Jorge Barbazan, Anthony Simon, Danijela Matic Vignjevic, Denis Krndija

    Pages 163-173

15. Intravital Imaging of Tumor Cell Motility in the Tumor Microenvironment Context

    • Battuya Bayarmagnai, Louisiane Perrin, Kamyar Esmaeili Pourfarhangi, Bojana Gligorijevic

    Pages 175-193

16. Using the Zebrafish Embryo to Dissect the Early Steps of the Metastasis Cascade

    • Gautier Follain, Naël Osmani, Cédric Fuchs, Guillaume Allio, Sébastien Harlepp, Jacky G. Goetz

    Pages 195-211

17. Analysis of In Vivo Cell Migration in Mosaic Zebrafish Embryos

    • Arthur Boutillon, Florence A. Giger, Nicolas B. David

    Pages 213-226

18. Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo

    • Marike Rüder, Benedikt M. Nagel, Sven Bogdan

    Pages 227-238

19. Migration of Q Cells in Caenorhabditis elegans

    • Yongping Chai, Zhiwen Zhu, Guangshuo Ou

    Pages 239-255

20. Imaging the Molecular Machines That Power Cell Migration

    • Anika Steffen, Frieda Kage, Klemens Rottner

    Pages 257-277

This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field.

• mammalian cells
• High throughput analysis
• Biosensors
• membrane protrusion
• Optogenetic

“This book presents several techniques to study cells migration in vivo, in vitro, ex vivo and with different model systems to dissect many of the biochemical and biophysical properties (at both molecular and cellular levels) involved in the dynamics of migration and cell-to-cell communication. … the editor of this book did a great job in collecting contributions where several animal models are presented.” (Manuela Monti, European Journal of Histochemistry, Vol. 63 (01), 2019)​

• Laboratory of Biochemistry, Ecole Polytechnique, Palaiseau, France

  Alexis Gautreau

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