- Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts
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- About this book
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This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field.
- Reviews
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“This book presents several techniques to study cells migration in vivo, in vitro, ex vivo and with different model systems to dissect many of the biochemical and biophysical properties (at both molecular and cellular levels) involved in the dynamics of migration and cell-to-cell communication. … the editor of this book did a great job in collecting contributions where several animal models are presented.” (Manuela Monti, European Journal of Histochemistry, Vol. 63 (01), 2019)
- Table of contents (28 chapters)
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Random Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories
Pages 1-9
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Directional Collective Migration in Wound Healing Assays
Pages 11-19
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An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition
Pages 21-27
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An In Vitro System to Study the Epithelial–Mesenchymal Transition In Vitro
Pages 29-42
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Detection of Migrasomes
Pages 43-49
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Table of contents (28 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Cell Migration
- Book Subtitle
- Methods and Protocols
- Editors
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- Alexis Gautreau
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1749
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7701-7
- DOI
- 10.1007/978-1-4939-7701-7
- Hardcover ISBN
- 978-1-4939-7700-0
- Softcover ISBN
- 978-1-4939-9261-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 405
- Number of Illustrations
- 10 b/w illustrations, 88 illustrations in colour
- Topics
