Methods in Molecular Biology

Sertoli Cells

Methods and Protocols

Editors: Alves, Marco G., Oliveira, Pedro F. (Eds.)

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  • Includes cutting-edge techniques for the study and use of Sertoli cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-4939-7698-0
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Hardcover $139.99
price for USA in USD
  • ISBN 978-1-4939-7697-3
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.99
price for USA in USD
  • ISBN 978-1-4939-8543-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.

Reviews

“The pictures illustrating the book are all of high quality and the readers will be satisfied and certainly not disappointed while running through the chapters: exactly in the same manner you cannot stop of eating chapters or cherries, one is calling the other!” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 63 (01), 2019)​

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Establishment of Primary Culture of Sertoli Cells

    Pages 1-8

    Bernardino, Raquel L. (et al.)

  • Evaluation of the Purity of Sertoli Cell Primary Cultures

    Pages 9-15

    Bernardino, Raquel L. (et al.)

  • Preparation of Testicular Samples for Histology and Immunohistochemistry

    Pages 17-36

    Bilinska, Barbara (et al.)

  • Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age

    Pages 37-47

    Grieco, Valeria (et al.)

  • Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy

    Pages 49-60

    Martínez-Hernández, Jesús (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7698-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
  • ISBN 978-1-4939-7697-3
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.99
price for USA in USD
  • ISBN 978-1-4939-8543-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Sertoli Cells
Book Subtitle
Methods and Protocols
Editors
  • Marco G. Alves
  • Pedro F. Oliveira
Series Title
Methods in Molecular Biology
Series Volume
1748
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7698-0
DOI
10.1007/978-1-4939-7698-0
Hardcover ISBN
978-1-4939-7697-3
Softcover ISBN
978-1-4939-8543-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 297
Number of Illustrations
12 b/w illustrations, 37 illustrations in colour
Topics