Sertoli Cells
Methods and Protocols
Editors: Alves, Marco G., Oliveira, Pedro F. (Eds.)
Free Preview- Includes cutting-edge techniques for the study and use of Sertoli cells
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells. - Reviews
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“The pictures illustrating the book are all of high quality and the readers will be satisfied and certainly not disappointed while running through the chapters: exactly in the same manner you cannot stop of eating chapters or cherries, one is calling the other!” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 63 (01), 2019)
- Table of contents (20 chapters)
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Establishment of Primary Culture of Sertoli Cells
Pages 1-8
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Evaluation of the Purity of Sertoli Cell Primary Cultures
Pages 9-15
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Preparation of Testicular Samples for Histology and Immunohistochemistry
Pages 17-36
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Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age
Pages 37-47
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Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy
Pages 49-60
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Sertoli Cells
- Book Subtitle
- Methods and Protocols
- Editors
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- Marco G. Alves
- Pedro F. Oliveira
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1748
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7698-0
- DOI
- 10.1007/978-1-4939-7698-0
- Hardcover ISBN
- 978-1-4939-7697-3
- Softcover ISBN
- 978-1-4939-8543-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 297
- Number of Illustrations
- 12 b/w illustrations, 37 illustrations in colour
- Topics
