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Methods in Molecular Biology

Hypoxia

Methods and Protocols

Editors: Huang, L Eric (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7665-2
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Softcover $139.00
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About this book

This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Hypoxia: Methods and Protocols aims to provide a valuable set of tools that can be used to study hypoxia and beyond.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Genetic Knockdown and Pharmacologic Inhibition of Hypoxia-Inducible Factor (HIF) Hydroxylases

    Pages 1-14

    Pickel, Christina (et al.)

  • Kinetic Analysis of HIF Prolyl Hydroxylases

    Pages 15-25

    Koivunen, Peppi (et al.)

  • Mass Spectrometry and Bioinformatic Analysis of Hydroxylation-Dependent Protein-Protein Interactions

    Pages 27-36

    Rodriguez, Javier (et al.)

  • Acquisition of Temporal HIF Transcriptional Activity Using a Secreted Luciferase Assay

    Pages 37-44

    Cavadas, Miguel A. S. (et al.)

  • Fluorescence Lifetime Imaging Microscopy (FLIM) as a Tool to Investigate Hypoxia-Induced Protein-Protein Interaction in Living Cells

    Pages 45-53

    Schützhold, Vera (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-7665-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA in USD
  • ISBN 978-1-4939-7664-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8538-8
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hypoxia
Book Subtitle
Methods and Protocols
Editors
  • L Eric Huang
Series Title
Methods in Molecular Biology
Series Volume
1742
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7665-2
DOI
10.1007/978-1-4939-7665-2
Hardcover ISBN
978-1-4939-7664-5
Softcover ISBN
978-1-4939-8538-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 302
Number of Illustrations
10 b/w illustrations, 63 illustrations in colour
Topics

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