- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Hypoxia: Methods and Protocols aims to provide a valuable set of tools that can be used to study hypoxia and beyond.
- Table of contents (26 chapters)
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Genetic Knockdown and Pharmacologic Inhibition of Hypoxia-Inducible Factor (HIF) Hydroxylases
Pages 1-14
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Kinetic Analysis of HIF Prolyl Hydroxylases
Pages 15-25
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Mass Spectrometry and Bioinformatic Analysis of Hydroxylation-Dependent Protein-Protein Interactions
Pages 27-36
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Acquisition of Temporal HIF Transcriptional Activity Using a Secreted Luciferase Assay
Pages 37-44
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Fluorescence Lifetime Imaging Microscopy (FLIM) as a Tool to Investigate Hypoxia-Induced Protein-Protein Interaction in Living Cells
Pages 45-53
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Hypoxia
- Book Subtitle
- Methods and Protocols
- Editors
-
- L Eric Huang
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1742
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7665-2
- DOI
- 10.1007/978-1-4939-7665-2
- Hardcover ISBN
- 978-1-4939-7664-5
- Softcover ISBN
- 978-1-4939-8538-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 302
- Number of Illustrations
- 10 b/w illustrations, 63 illustrations in colour
- Topics
